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dc.contributor.authorBoyle, Patrick
dc.contributor.authorClement, Kendell
dc.contributor.authorGu, Hongcang
dc.contributor.authorSmith, Zachary D
dc.contributor.authorZiller, Michael Johannes
dc.contributor.authorFostel, Jennifer L
dc.contributor.authorHolmes, Laurie
dc.contributor.authorMeldrim, Jim
dc.contributor.authorKelley, Fontina
dc.contributor.authorGnirke, Andreas
dc.contributor.authorMeissner, Alexander
dc.date.accessioned2014-02-21T18:07:28Z
dc.date.issued2012
dc.identifier.citationBoyle, Patrick, Kendell Clement, Hongcang Gu, Zachary D Smith, Michael Ziller, Jennifer L Fostel, Laurie Holmes, et al. 2012. Gel-free multiplexed reduced representation bisulfite sequencing for large-scale dna methylation profiling. Genome Biology 13(10): R92.en_US
dc.identifier.issn1465-6906en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11729537
dc.description.abstractSequencing-based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of DNA methylation have been employed, throughput has been a key limitation for most. To further facilitate the mapping of DNA methylation, we describe a protocol for gel-free multiplexed reduced representation bisulfite sequencing (mRRBS) that reduces the workload dramatically and enables processing of 96 or more samples per week. mRRBS achieves similar CpG coverage to the original RRBS protocol, while the higher throughput and lower cost make it better suited for large-scale DNA methylation mapping studies, including cohorts of cancer samples.en_US
dc.description.sponsorshipStem Cell and Regenerative Biologyen_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/gb-2012-13-10-r92en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3491420/pdf/en_US
dash.licenseLAA
dc.titleGel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profilingen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalGenome Biologyen_US
dash.depositing.authorMeissner, Alexander
dc.date.available2014-02-21T18:07:28Z
dc.identifier.doi10.1186/gb-2012-13-10-r92*
dash.authorsorderedfalse
dash.contributor.affiliatedSmith, Zachary
dash.contributor.affiliatedZiller, Michael
dash.contributor.affiliatedBoyle, Patrick
dash.contributor.affiliatedMeissner, Alexander


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