Crystal structure of xenotropic murine leukaemia virus-related virus (XMRV) ribonuclease H

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Kim, Ju Hee
Kang, Sunghyun
Jung, Suk-Kyeong
Yu, Keum Ran
Chung, Sang J.
Chung, Bong Hyun
Kim, Bo Yeon
Kim, Seung Jun
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https://doi.org/10.1042/BSR20120028Metadata
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Kim, Ju Hee, Sunghyun Kang, Suk-Kyeong Jung, Keum Ran Yu, Sang J. Chung, Bong Hyun Chung, Raymond L. Erikson, Bo Yeon Kim, and Seung Jun Kim. 2012. Crystal structure of xenotropic murine leukaemia virus-related virus (xmrv) ribonuclease h. Bioscience Reports 32(Pt 5): 455-463.Abstract
RNase H (retroviral ribonuclease H) cleaves the phosphate backbone of the RNA template within an RNA/DNA hybrid to complete the synthesis of double-stranded viral DNA. In the present study we have determined the complete structure of the RNase H domain from XMRV (xenotropic murine leukaemia virus-related virus) RT (reverse transcriptase). The basic protrusion motif of the XMRV RNase H domain is folded as a short helix and an adjacent highly bent loop. Structural superposition and subsequent mutagenesis experiments suggest that the basic protrusion motif plays a role in direct binding to the major groove in RNA/DNA hybrid, as well as in establishing the co-ordination among modules in RT necessary for proper function.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475449/pdf/Terms of Use
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