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dc.contributor.authorMracek, Philipp
dc.contributor.authorSantoriello, Cristina
dc.contributor.authorIdda, M. Laura
dc.contributor.authorPagano, Cristina
dc.contributor.authorBen-Moshe, Zohar
dc.contributor.authorGothilf, Yoav
dc.contributor.authorVallone, Daniela
dc.contributor.authorFoulkes, Nicholas S.
dc.date.accessioned2014-02-24T21:20:46Z
dc.date.issued2012
dc.identifier.citationMracek, Philipp, Cristina Santoriello, M. Laura Idda, Cristina Pagano, Zohar Ben-Moshe, Yoav Gothilf, Daniela Vallone, and Nicholas S. Foulkes. 2012. Regulation of per and cry genes reveals a central role for the D-box enhancer in light-dependent gene expression. PLoS ONE 7(12): e51278.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11738407
dc.description.abstractLight serves as a key environmental signal for synchronizing the circadian clock with the day night cycle. The zebrafish represents an attractive model for exploring how light influences the vertebrate clock mechanism. Direct illumination of most fish tissues and cell lines induces expression of a broad range of genes including DNA repair, stress response and key clock genes. We have previously identified D- and E-box elements within the promoter of the zebrafish per2 gene that together direct light-induced gene expression. However, is the combined regulation by E- and D-boxes a general feature for all light-induced gene expression? We have tackled this question by examining the regulation of additional light-inducible genes. Our results demonstrate that with the exception of per2, all other genes tested are not induced by light upon blocking of de novo protein synthesis. We reveal that a single D-box serves as the principal light responsive element within the cry1a promoter. Furthermore, upon inhibition of protein synthesis D-box mediated gene expression is abolished while the E-box confers light driven activation as observed in the per2 gene. Given the existence of different photoreceptors in fish cells, our results implicate the D-box enhancer as a general convergence point for light driven signaling.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0051278en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516543/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectPhysiological Processesen_US
dc.subjectChronobiologyen_US
dc.subjectComputational Biologyen_US
dc.subjectMolecular Geneticsen_US
dc.subjectGene Regulationen_US
dc.subjectGene Expressionen_US
dc.subjectGeneticsen_US
dc.subjectModel Organismsen_US
dc.subjectAnimal Modelsen_US
dc.subjectZebrafishen_US
dc.subjectZoologyen_US
dc.subjectAnimal Physiologyen_US
dc.subjectPhysiologyen_US
dc.subjectAnatomyen_US
dc.titleRegulation of per and cry Genes Reveals a Central Role for the D-Box Enhancer in Light-Dependent Gene Expressionen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorSantoriello, Cristina
dc.date.available2014-02-24T21:20:46Z
dc.identifier.doi10.1371/journal.pone.0051278*
dash.contributor.affiliatedSantoriello, Cristina


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