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dc.contributor.advisorvan Oijen, Antoine
dc.contributor.authorOtterstrom, Jason John
dc.date.accessioned2014-02-25T18:55:23Z
dash.embargo.terms2015-02-04en_US
dash.embargo.terms2015-02-04
dc.date.issued2014-02-25
dc.date.submitted2013
dc.identifier.citationOtterstrom, Jason John. 2013. Visualizing Influenza Virus Membrane Fusion: Inhibition and Kinetics. Doctoral dissertation, Harvard University.en_US
dc.identifier.otherhttp://dissertations.umi.com/gsas.harvard:11251en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11744457
dc.description.abstractThe influenza virus hemagglutinin (HA) surface protein is a primary antigenic target for neutralization of viral infection. HA also mediates membrane fusion between the virus and a cell, which is the first critical step during infection. Traditional techniques to study infection neutralization by antibodies or the membrane fusion process rely on ensemble measurements, confounding the precise mechanism of infection neutralization and obscuring transient conformational intermediates. This dissertation describes advances made in a fluorescence microscopy-based single-particle fusion assay to overcome the limitations of ensemble measurements in these types of studies. Virus particles are labeled to visualize lipid mixing between a virus and a target membrane formed upon a glass or polymer support. Optionally, the viral lumen can be labeled to visualize the subsequent release of viral contents.en_US
dc.language.isoen_USen_US
dash.licenseLAA
dc.subjectBiophysicsen_US
dc.subjectVirologyen_US
dc.subjectHemagglutininen_US
dc.subjectInfluenzaen_US
dc.subjectMembrane Fusionen_US
dc.subjectNeutralization Stoichiometryen_US
dc.subjectNeutralizing Antibodiesen_US
dc.subjectSingle Molecule Biophysicsen_US
dc.titleVisualizing Influenza Virus Membrane Fusion: Inhibition and Kineticsen_US
dc.typeThesis or Dissertationen_US
dash.depositing.authorOtterstrom, Jason John
dc.date.available2015-02-04T08:30:41Z
thesis.degree.date2013en_US
thesis.degree.disciplineBiophysicsen_US
thesis.degree.grantorHarvard Universityen_US
thesis.degree.leveldoctoralen_US
thesis.degree.namePh.D.en_US
dc.contributor.committeeMemberHogle, Jamesen_US
dc.contributor.committeeMemberLoparo, Josephen_US
dc.contributor.committeeMemberZhuang, Xiaoweien_US
dash.contributor.affiliatedOtterstrom, Jason John


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