dc.contributor.advisor | Farzan, Michael | |
dc.contributor.author | Quinlan, Brian Donald | |
dc.date.accessioned | 2014-02-25T21:51:38Z | |
dc.date.issued | 2014-02-25 | |
dc.date.submitted | 2013 | |
dc.identifier.citation | Quinlan, Brian Donald. 2014. Directed Evolution of Peptide Inhibitors of HIV-1 Entry. Doctoral dissertation, Harvard University. | en_US |
dc.identifier.other | http://dissertations.umi.com/gsas.harvard:11297 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11745713 | |
dc.description.abstract | The conflict between HIV-1 and the host immune system plays out over a time-scale of months and years, and on a grander scale in the co-evolution of lentiviruses and the immune systems of their host species. Directed evolution of HIV-1 entry inhibitors using controlled randomization together with a display system offers a means of recapitulating one side of this conflict in vitro on an accelerated time-scale. To address limitations in existing display systems, we constructed a vector (pDQ1) integrating phage-display and mammalian-expression systems. This vector displays on phage when expressed in bacteria, and as an Fc-fusion when expressed in tissue culture, thus accelerating the iterative process of randomization, display, and characterization. We demonstrated the utility of this vector in the evolution of a CD4-mimetic peptide. | en_US |
dc.language.iso | en_US | en_US |
dash.license | LAA | |
dc.subject | Virology | en_US |
dc.subject | directed evolution | en_US |
dc.subject | HIV | en_US |
dc.subject | peptide inhibitors | en_US |
dc.subject | Phage display | en_US |
dc.subject | protein maturation | en_US |
dc.subject | receptor mimetic peptides | en_US |
dc.title | Directed Evolution of Peptide Inhibitors of HIV-1 Entry | en_US |
dc.type | Thesis or Dissertation | en_US |
dash.depositing.author | Quinlan, Brian Donald | |
dc.date.available | 2014-02-25T21:51:38Z | |
thesis.degree.date | 2014 | en_US |
thesis.degree.discipline | Biology: Medical Sciences, Division of | en_US |
thesis.degree.grantor | Harvard University | en_US |
thesis.degree.level | doctoral | en_US |
thesis.degree.name | Ph.D. | en_US |
dc.contributor.committeeMember | Marasco, Wayne | en_US |
dc.contributor.committeeMember | Gabuzda, Dana | en_US |
dc.contributor.committeeMember | Engelman, Alan | en_US |
dc.contributor.committeeMember | Clapham, Paul | en_US |
dash.contributor.affiliated | Quinlan, Brian Donald | |