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dc.contributor.authorCarlson, Alicia L.en_US
dc.contributor.authorFujisaki, Jojien_US
dc.contributor.authorWu, Juwellen_US
dc.contributor.authorRunnels, Judith M.en_US
dc.contributor.authorTurcotte, Raphaëlen_US
dc.contributor.authorCelso, Cristina Loen_US
dc.contributor.authorScadden, David T.en_US
dc.contributor.authorStrom, Terry B.en_US
dc.contributor.authorLin, Charles P.en_US
dc.date.accessioned2014-03-01T02:23:39Z
dc.date.issued2013en_US
dc.identifier.citationCarlson, Alicia L., Joji Fujisaki, Juwell Wu, Judith M. Runnels, Raphaël Turcotte, Cristina Lo Celso, David T. Scadden, Terry B. Strom, and Charles P. Lin. 2013. “Tracking Single Cells in Live Animals Using a Photoconvertible Near-Infrared Cell Membrane Label.” PLoS ONE 8 (8): e69257. doi:10.1371/journal.pone.0069257. http://dx.doi.org/10.1371/journal.pone.0069257.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11855713
dc.description.abstractWe describe a novel photoconversion technique to track individual cells in vivo using a commercial lipophilic membrane dye, DiR. We show that DiR exhibits a permanent fluorescence emission shift (photoconversion) after light exposure and does not reacquire the original color over time. Ratiometric imaging can be used to distinguish photoconverted from non-converted cells with high sensitivity. Combining the use of this photoconvertible dye with intravital microscopy, we tracked the division of individual hematopoietic stem/progenitor cells within the calvarium bone marrow of live mice. We also studied the peripheral differentiation of individual T cells by tracking the gain or loss of FoxP3-GFP expression, a marker of the immune suppressive function of CD4+ T cells. With the near-infrared photoconvertible membrane dye, the entire visible spectral range is available for simultaneous use with other fluorescent proteins to monitor gene expression or to trace cell lineage commitment in vivo with high spatial and temporal resolution.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0069257en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753322/pdf/en
dash.licenseLAAen_US
dc.subjectBiologyen
dc.subjectBiotechnologyen
dc.subjectBioengineeringen
dc.subjectBiomedical Engineeringen
dc.subjectDevelopmental Biologyen
dc.subjectStem Cellsen
dc.subjectHematopoietic Stem Cellsen
dc.subjectStem Cell Nicheen
dc.subjectImmunologyen
dc.subjectImmune Cellsen
dc.subjectT Cellsen
dc.subjectModel Organismsen
dc.subjectAnimal Modelsen
dc.subjectMolecular Cell Biologyen
dc.subjectCellular Typesen
dc.subjectEngineeringen
dc.subjectMedicineen
dc.subjectHematologyen
dc.subjectBone Marrow and Stem Cell Transplantationen
dc.subjectPhysicsen
dc.subjectCondensed-Matter Physicsen
dc.subjectOpticsen
dc.titleTracking Single Cells in Live Animals Using a Photoconvertible Near-Infrared Cell Membrane Labelen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorWu, Juwellen_US
dc.date.available2014-03-01T02:23:39Z
dc.identifier.doi10.1371/journal.pone.0069257*
dash.contributor.affiliatedWu, Juwell
dash.contributor.affiliatedScadden, David
dash.contributor.affiliatedStrom, Terry B.
dash.contributor.affiliatedLin, Charles


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