dc.contributor.author | Bohl, Christopher | en_US |
dc.contributor.author | Bowder, Dane | en_US |
dc.contributor.author | Thompson, Jesse | en_US |
dc.contributor.author | Abrahamyan, Levon | en_US |
dc.contributor.author | Gonzalez-Ramirez, Sandra | en_US |
dc.contributor.author | Mao, Youdong | en_US |
dc.contributor.author | Sodroski, Joseph | en_US |
dc.contributor.author | Wood, Charles | en_US |
dc.contributor.author | Xiang, Shi-hua | en_US |
dc.date.accessioned | 2014-03-01T02:23:59Z | |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Bohl, Christopher, Dane Bowder, Jesse Thompson, Levon Abrahamyan, Sandra Gonzalez-Ramirez, Youdong Mao, Joseph Sodroski, Charles Wood, and Shi-hua Xiang. 2013. “A Twin-Cysteine Motif in the V2 Region of gp120 Is Associated with SIV Envelope Trimer Stabilization.” PLoS ONE 8 (7): e69406. doi:10.1371/journal.pone.0069406. http://dx.doi.org/10.1371/journal.pone.0069406. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11855743 | |
dc.description.abstract | The V1 and V2 variable regions of the primate immunodeficiency viruses contribute to the trimer association domain of the gp120 exterior envelope glycoprotein. A pair of V2 cysteine residues at 183 and 191 (“twin cysteines”) is present in several simian immunodeficiency viruses, human immunodeficiency virus type 2 (HIV-2) and some SIVcpz lineages, but not in HIV-1. To examine the role of this potentially disulfide-bonded twin-cysteine motif, the cysteine residues in the SIVmac239 envelope glycoproteins were individually and pairwise substituted by alanine residues. All of the twin-cysteine mutants exhibited decreases in gp120 association with the Env trimer, membrane-fusing activity, and ability to support virus entry. Thus, the twin-cysteine motif plays a role in Env trimer stabilization in SIV and may do so in HIV-2 and some SIVcpz as well. This implies that HIV-1 lost the twin-cysteines, and may have relatively unstable Env trimers compared to SIV and HIV-2. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0069406 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720275/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Biology | en |
dc.subject | Immunology | en |
dc.subject | Immune Response | en |
dc.subject | Microbiology | en |
dc.subject | Virology | en |
dc.subject | Viral Classification | en |
dc.subject | Retroviruses | en |
dc.subject | Viral Structure | en |
dc.subject | Viral Envelope | en |
dc.subject | Immunodeficiency Viruses | en |
dc.subject | Viral Evolution | en |
dc.subject | Viral Immune Evasion | en |
dc.subject | Viral Vaccines | en |
dc.subject | Molecular Cell Biology | en |
dc.title | A Twin-Cysteine Motif in the V2 Region of gp120 Is Associated with SIV Envelope Trimer Stabilization | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Mao, Youdong | en_US |
dc.date.available | 2014-03-01T02:23:59Z | |
dc.identifier.doi | 10.1371/journal.pone.0069406 | * |
dash.contributor.affiliated | Mao, Youdong | |
dash.contributor.affiliated | Sodroski, Joseph | |