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dc.contributor.authorBohl, Christopheren_US
dc.contributor.authorBowder, Daneen_US
dc.contributor.authorThompson, Jesseen_US
dc.contributor.authorAbrahamyan, Levonen_US
dc.contributor.authorGonzalez-Ramirez, Sandraen_US
dc.contributor.authorMao, Youdongen_US
dc.contributor.authorSodroski, Josephen_US
dc.contributor.authorWood, Charlesen_US
dc.contributor.authorXiang, Shi-huaen_US
dc.date.accessioned2014-03-01T02:23:59Z
dc.date.issued2013en_US
dc.identifier.citationBohl, Christopher, Dane Bowder, Jesse Thompson, Levon Abrahamyan, Sandra Gonzalez-Ramirez, Youdong Mao, Joseph Sodroski, Charles Wood, and Shi-hua Xiang. 2013. “A Twin-Cysteine Motif in the V2 Region of gp120 Is Associated with SIV Envelope Trimer Stabilization.” PLoS ONE 8 (7): e69406. doi:10.1371/journal.pone.0069406. http://dx.doi.org/10.1371/journal.pone.0069406.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11855743
dc.description.abstractThe V1 and V2 variable regions of the primate immunodeficiency viruses contribute to the trimer association domain of the gp120 exterior envelope glycoprotein. A pair of V2 cysteine residues at 183 and 191 (“twin cysteines”) is present in several simian immunodeficiency viruses, human immunodeficiency virus type 2 (HIV-2) and some SIVcpz lineages, but not in HIV-1. To examine the role of this potentially disulfide-bonded twin-cysteine motif, the cysteine residues in the SIVmac239 envelope glycoproteins were individually and pairwise substituted by alanine residues. All of the twin-cysteine mutants exhibited decreases in gp120 association with the Env trimer, membrane-fusing activity, and ability to support virus entry. Thus, the twin-cysteine motif plays a role in Env trimer stabilization in SIV and may do so in HIV-2 and some SIVcpz as well. This implies that HIV-1 lost the twin-cysteines, and may have relatively unstable Env trimers compared to SIV and HIV-2.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0069406en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720275/pdf/en
dash.licenseLAAen_US
dc.subjectBiologyen
dc.subjectImmunologyen
dc.subjectImmune Responseen
dc.subjectMicrobiologyen
dc.subjectVirologyen
dc.subjectViral Classificationen
dc.subjectRetrovirusesen
dc.subjectViral Structureen
dc.subjectViral Envelopeen
dc.subjectImmunodeficiency Virusesen
dc.subjectViral Evolutionen
dc.subjectViral Immune Evasionen
dc.subjectViral Vaccinesen
dc.subjectMolecular Cell Biologyen
dc.titleA Twin-Cysteine Motif in the V2 Region of gp120 Is Associated with SIV Envelope Trimer Stabilizationen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorMao, Youdongen_US
dc.date.available2014-03-01T02:23:59Z
dc.identifier.doi10.1371/journal.pone.0069406*
dash.contributor.affiliatedSodroski, Joseph
dash.contributor.affiliatedMao, Youdong


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