Frequent Intra-Subtype Recombination among HIV-1 Circulating in Tanzania

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Frequent Intra-Subtype Recombination among HIV-1 Circulating in Tanzania

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dc.contributor.author Kiwelu, Ireen E. en_US
dc.contributor.author Novitsky, Vladimir en_US
dc.contributor.author Margolin, Lauren en_US
dc.contributor.author Baca, Jeannie en_US
dc.contributor.author Manongi, Rachel en_US
dc.contributor.author Sam, Noel en_US
dc.contributor.author Shao, John en_US
dc.contributor.author McLane, Mary F. en_US
dc.contributor.author Kapiga, Saidi H. en_US
dc.contributor.author Essex, M. en_US
dc.date.accessioned 2014-03-01T02:24:08Z
dc.date.issued 2013 en_US
dc.identifier.citation Kiwelu, Ireen E., Vladimir Novitsky, Lauren Margolin, Jeannie Baca, Rachel Manongi, Noel Sam, John Shao, Mary F. McLane, Saidi H. Kapiga, and M. Essex. 2013. “Frequent Intra-Subtype Recombination among HIV-1 Circulating in Tanzania.” PLoS ONE 8 (8): e71131. doi:10.1371/journal.pone.0071131. http://dx.doi.org/10.1371/journal.pone.0071131. en
dc.identifier.issn 1932-6203 en
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:11855755
dc.description.abstract The study estimated the prevalence of HIV-1 intra-subtype recombinant variants among female bar and hotel workers in Tanzania. While intra-subtype recombination occurs in HIV-1, it is generally underestimated. HIV-1 env gp120 V1-C5 quasispecies from 45 subjects were generated by single-genome amplification and sequencing (median (IQR) of 38 (28–50) sequences per subject). Recombination analysis was performed using seven methods implemented within the recombination detection program version 3, RDP3. HIV-1 sequences were considered recombinant if recombination signals were detected by at least three methods with p-values of ≤0.05 after Bonferroni correction for multiple comparisons. HIV-1 in 38 (84%) subjects showed evidence for intra-subtype recombination including 22 with HIV-1 subtype A1, 13 with HIV-1 subtype C, and 3 with HIV-1 subtype D. The distribution of intra-patient recombination breakpoints suggested ongoing recombination and showed selective enrichment of recombinant variants in 23 (60%) subjects. The number of subjects with evidence of intra-subtype recombination increased from 29 (69%) to 36 (82%) over one year of follow-up, although the increase did not reach statistical significance. Adjustment for intra-subtype recombination is important for the analysis of multiplicity of HIV infection. This is the first report of high prevalence of intra-subtype recombination in the HIV/AIDS epidemic in Tanzania, a region where multiple HIV-1 subtypes co-circulate. HIV-1 intra-subtype recombination increases viral diversity and presents additional challenges for HIV-1 vaccine design. en
dc.language.iso en_US en
dc.publisher Public Library of Science en
dc.relation.isversionof doi:10.1371/journal.pone.0071131 en
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733632/pdf/ en
dash.license LAA en_US
dc.subject Biology en
dc.subject Population Biology en
dc.subject Epidemiology en
dc.subject Infectious Disease Epidemiology en
dc.subject Medicine en
dc.subject Clinical Research Design en
dc.subject Cohort Studies en
dc.subject Prospective Studies en
dc.subject Infectious Diseases en
dc.subject Viral Diseases en
dc.subject HIV en
dc.subject HIV epidemiology en
dc.subject Retrovirology and HIV immunopathogenesis en
dc.subject Obstetrics and Gynecology en
dc.subject Genitourinary Infections en
dc.subject Urology en
dc.title Frequent Intra-Subtype Recombination among HIV-1 Circulating in Tanzania en
dc.type Journal Article en_US
dc.description.version Version of Record en
dc.relation.journal PLoS ONE en
dash.depositing.author Novitsky, Vladimir en_US
dc.date.available 2014-03-01T02:24:08Z

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