Show simple item record

dc.contributor.authorKiwelu, Ireen E.en_US
dc.contributor.authorNovitsky, Vladimiren_US
dc.contributor.authorMargolin, Laurenen_US
dc.contributor.authorBaca, Jeannieen_US
dc.contributor.authorManongi, Rachelen_US
dc.contributor.authorSam, Noelen_US
dc.contributor.authorShao, Johnen_US
dc.contributor.authorMcLane, Mary F.en_US
dc.contributor.authorKapiga, Saidi H.en_US
dc.contributor.authorEssex, M.en_US
dc.date.accessioned2014-03-01T02:24:08Z
dc.date.issued2013en_US
dc.identifier.citationKiwelu, Ireen E., Vladimir Novitsky, Lauren Margolin, Jeannie Baca, Rachel Manongi, Noel Sam, John Shao, Mary F. McLane, Saidi H. Kapiga, and M. Essex. 2013. “Frequent Intra-Subtype Recombination among HIV-1 Circulating in Tanzania.” PLoS ONE 8 (8): e71131. doi:10.1371/journal.pone.0071131. http://dx.doi.org/10.1371/journal.pone.0071131.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11855755
dc.description.abstractThe study estimated the prevalence of HIV-1 intra-subtype recombinant variants among female bar and hotel workers in Tanzania. While intra-subtype recombination occurs in HIV-1, it is generally underestimated. HIV-1 env gp120 V1-C5 quasispecies from 45 subjects were generated by single-genome amplification and sequencing (median (IQR) of 38 (28–50) sequences per subject). Recombination analysis was performed using seven methods implemented within the recombination detection program version 3, RDP3. HIV-1 sequences were considered recombinant if recombination signals were detected by at least three methods with p-values of ≤0.05 after Bonferroni correction for multiple comparisons. HIV-1 in 38 (84%) subjects showed evidence for intra-subtype recombination including 22 with HIV-1 subtype A1, 13 with HIV-1 subtype C, and 3 with HIV-1 subtype D. The distribution of intra-patient recombination breakpoints suggested ongoing recombination and showed selective enrichment of recombinant variants in 23 (60%) subjects. The number of subjects with evidence of intra-subtype recombination increased from 29 (69%) to 36 (82%) over one year of follow-up, although the increase did not reach statistical significance. Adjustment for intra-subtype recombination is important for the analysis of multiplicity of HIV infection. This is the first report of high prevalence of intra-subtype recombination in the HIV/AIDS epidemic in Tanzania, a region where multiple HIV-1 subtypes co-circulate. HIV-1 intra-subtype recombination increases viral diversity and presents additional challenges for HIV-1 vaccine design.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0071131en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733632/pdf/en
dash.licenseLAAen_US
dc.subjectBiologyen
dc.subjectPopulation Biologyen
dc.subjectEpidemiologyen
dc.subjectInfectious Disease Epidemiologyen
dc.subjectMedicineen
dc.subjectClinical Research Designen
dc.subjectCohort Studiesen
dc.subjectProspective Studiesen
dc.subjectInfectious Diseasesen
dc.subjectViral Diseasesen
dc.subjectHIVen
dc.subjectHIV epidemiologyen
dc.subjectRetrovirology and HIV immunopathogenesisen
dc.subjectObstetrics and Gynecologyen
dc.subjectGenitourinary Infectionsen
dc.subjectUrologyen
dc.titleFrequent Intra-Subtype Recombination among HIV-1 Circulating in Tanzaniaen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorNovitsky, Vladimiren_US
dc.date.available2014-03-01T02:24:08Z
dc.identifier.doi10.1371/journal.pone.0071131*
dash.contributor.affiliatedMcLane, Mary
dash.contributor.affiliatedNovitsky, Vladimir


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record