dc.contributor.author | Riedel, Christian G. | en_US |
dc.contributor.author | Dowen, Robert H. | en_US |
dc.contributor.author | Lourenco, Guinevere F. | en_US |
dc.contributor.author | Kirienko, Natalia V. | en_US |
dc.contributor.author | Heimbucher, Thomas | en_US |
dc.contributor.author | West, Jason A. | en_US |
dc.contributor.author | Bowman, Sarah K. | en_US |
dc.contributor.author | Kingston, Robert E. | en_US |
dc.contributor.author | Dillin, Andrew | en_US |
dc.contributor.author | Asara, John M. | en_US |
dc.contributor.author | Ruvkun, Gary | en_US |
dc.date.accessioned | 2014-03-01T02:25:06Z | |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Riedel, C. G., R. H. Dowen, G. F. Lourenco, N. V. Kirienko, T. Heimbucher, J. A. West, S. K. Bowman, et al. 2013. “DAF-16/FOXO employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity.” Nature cell biology 15 (5): 491-501. doi:10.1038/ncb2720. http://dx.doi.org/10.1038/ncb2720. | en |
dc.identifier.issn | 1465-7392 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11855858 | |
dc.description.abstract | Organisms are constantly challenged by stresses and privations and require adaptive responses for their survival. The transcription factor DAF-16/FOXO is central nexus in these responses, but despite its importance little is known about how it regulates its target genes. Proteomic identification of DAF-16/FOXO binding partners in Caenorhabditis elegans and their subsequent functional evaluation by RNA interference (RNAi) revealed several candidate DAF-16/FOXO cofactors, most notably the chromatin remodeller SWI/SNF. DAF-16/FOXO and SWI/SNF form a complex and globally colocalize at DAF-16/FOXO target promoters. We show that specifically for gene-activation, DAF-16/FOXO depends on SWI/SNF, facilitating SWI/SNF recruitment to target promoters, in order to activate transcription by presumed remodelling of local chromatin. For the animal, this translates into an essential role of SWI/SNF for DAF-16/FOXO-mediated processes, i.e. dauer formation, stress resistance, and the promotion of longevity. Thus we give insight into the mechanisms of DAF-16/FOXO-mediated transcriptional regulation and establish a critical link between ATP-dependent chromatin remodelling and lifespan regulation. | en |
dc.language.iso | en_US | en |
dc.relation.isversionof | doi:10.1038/ncb2720 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748955/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | | en |
dc.subject | FOXO | en |
dc.subject | SWI/SNF | en |
dc.subject | longevity | en |
dc.subject | stress response | en |
dc.subject | dauer formation | en |
dc.subject | chromatin remodelling | en |
dc.subject | proteomics | en |
dc.title | DAF-16/FOXO employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Nature cell biology | en |
dash.depositing.author | Dowen, Robert H. | en_US |
dc.date.available | 2014-03-01T02:25:06Z | |
dc.identifier.doi | 10.1038/ncb2720 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Bowman, Sarah K. | |
dash.contributor.affiliated | Kirienko, Natalia | |
dash.contributor.affiliated | Dowen, Robert H. | |
dash.contributor.affiliated | Kingston, Robert | |
dash.contributor.affiliated | Asara, John | |
dash.contributor.affiliated | Ruvkun, Gary | |