SnoN Facilitates Axonal Regeneration after Spinal Cord Injury

View/ Open
Author
Do, Jiun L.
Bonni, Azad
Tuszynski, Mark H.
Published Version
https://doi.org/10.1371/journal.pone.0071906Metadata
Show full item recordCitation
Do, Jiun L., Azad Bonni, and Mark H. Tuszynski. 2013. “SnoN Facilitates Axonal Regeneration after Spinal Cord Injury.” PLoS ONE 8 (8): e71906. doi:10.1371/journal.pone.0071906. http://dx.doi.org/10.1371/journal.pone.0071906.Abstract
Adult CNS neurons exhibit a reduced capacity for growth compared to developing neurons, due in part to downregulation of growth-associated genes as development is completed. We tested the hypothesis that SnoN, an embryonically regulated transcription factor that specifies growth of the axonal compartment, can enhance growth in injured adult neurons. In vitro, SnoN overexpression in dissociated adult DRG neuronal cultures significantly enhanced neurite outgrowth. Moreover, TGF-β1, a negative regulator of SnoN, inhibited neurite outgrowth, and SnoN over-expression overcame this inhibition. We then examined whether SnoN influenced axonal regeneration in vivo: indeed, expression of a mutant form of SnoN resistant to degradation significantly enhanced axonal regeneration following cervical spinal cord injury, despite peri-lesional upregulation of TGF-β1. Thus, a developmental mechanism that specifies extension of the axonal compartment also promotes axonal regeneration after adult CNS injury.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732222/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:11855930
Collections
- HMS Scholarly Articles [17875]
Contact administrator regarding this item (to report mistakes or request changes)