Show simple item record

dc.contributor.authorChen, Alice E.
dc.contributor.authorBorowiak, Malgorzata
dc.contributor.authorSherwood, Richard Irving
dc.contributor.authorKweudjeu, Anastasie
dc.contributor.authorMelton, Douglas A.
dc.date.accessioned2014-03-07T12:57:30Z
dc.date.issued2013
dc.identifierQuick submit: 2014-02-07T09:57:24-05:00
dc.identifier.citationChen, A. E., M. Borowiak, R. I. Sherwood, A. Kweudjeu, and D. A. Melton. 2013. “Functional Evaluation of ES Cell-Derived Endodermal Populations Reveals Differences Between Nodal and Activin A-Guided Differentiation.” Development 140 (3) (February 1): 675–686. doi:10.1242/dev.085431. http://dx.doi.org/10.1242/dev.085431.en_US
dc.identifier.issn0950-1991en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11870369
dc.description.abstractEmbryonic stem (ES) cells hold great promise with respect to their potential to be differentiated into desired cell types. Of interest are organs derived from the definitive endoderm, such as the pancreas and liver, and animal studies have revealed an essential role for Nodal in development of the definitive endoderm. Activin A is a related TGFβ member that acts through many of the same downstream signaling effectors as Nodal and is thought to mimic Nodal activity. Detailed characterization of ES cell-derived endodermal cell types by gene expression analysis in vitro and functional analysis in vivo reveal that, despite their similarity in gene expression, Nodal and Activin-derived endodermal cells exhibit a distinct difference in functional competence following transplantation into the developing mouse embryo. Pdx1-expressing cells arising from the respective endoderm populations exhibit extended differences in their competence to mature into insulin/c-peptide-expressing cells in vivo. Our findings underscore the importance of functional cell-type evaluation during stepwise differentiation of stem cells.en_US
dc.description.sponsorshipStem Cell and Regenerative Biologyen_US
dc.language.isoen_USen_US
dc.publisherThe Company of Biologistsen_US
dc.relation.isversionofdoi:10.1242/dev.085431en_US
dash.licenseLAA
dc.titleFunctional evaluation of ES cell-derived endodermal populations reveals differences between Nodal and Activin A-guided differentiationen_US
dc.typeJournal Articleen_US
dc.date.updated2014-02-07T14:58:57Z
dc.description.versionVersion of Recorden_US
dc.rights.holderChen AE, Borowiak, M, Sherwood R, Kweudjeu A, Melton, DA
dc.relation.journalDevelopmenten_US
dash.depositing.authorMelton, Douglas A.
dc.date.available2014-03-07T12:57:30Z
dc.identifier.doi10.1242/dev.085431*
workflow.legacycommentsIn this journal's terms <http://www.biologists.com/copyright_permissions.html#open> they say that articles under the Open option are CC-BY and will be made available immediately ON THEIR WEBSITE. This article, on the website, says "Free" next to the PDF and full text options; other articles, such as doi: 10.1242/dev.101915 do not, and lead me to a pay wall. Therefore, I conclude this item is CC-BY.en_US
dash.contributor.affiliatedSherwood, Richard
dash.contributor.affiliatedMelton, Douglas


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record