dc.contributor.author | Chen, Alice E. | |
dc.contributor.author | Borowiak, Malgorzata | |
dc.contributor.author | Sherwood, Richard Irving | |
dc.contributor.author | Kweudjeu, Anastasie | |
dc.contributor.author | Melton, Douglas A. | |
dc.date.accessioned | 2014-03-07T12:57:30Z | |
dc.date.issued | 2013 | |
dc.identifier | Quick submit: 2014-02-07T09:57:24-05:00 | |
dc.identifier.citation | Chen, A. E., M. Borowiak, R. I. Sherwood, A. Kweudjeu, and D. A. Melton. 2013. “Functional Evaluation of ES Cell-Derived Endodermal Populations Reveals Differences Between Nodal and Activin A-Guided Differentiation.” Development 140 (3) (February 1): 675–686. doi:10.1242/dev.085431. http://dx.doi.org/10.1242/dev.085431. | en_US |
dc.identifier.issn | 0950-1991 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11870369 | |
dc.description.abstract | Embryonic stem (ES) cells hold great promise with respect to their potential to be differentiated into desired cell types. Of interest are organs derived from the definitive endoderm, such as the pancreas and liver, and animal studies have revealed an essential role for Nodal in development of the definitive endoderm. Activin A is a related TGFβ member that acts through many of the same downstream signaling effectors as Nodal and is thought to mimic Nodal activity. Detailed characterization of ES cell-derived endodermal cell types by gene expression analysis in vitro and functional analysis in vivo reveal that, despite their similarity in gene expression, Nodal and Activin-derived endodermal cells exhibit a distinct difference in functional competence following transplantation into the developing mouse embryo. Pdx1-expressing cells arising from the respective endoderm populations exhibit extended differences in their competence to mature into insulin/c-peptide-expressing cells in vivo. Our findings underscore the importance of functional cell-type evaluation during stepwise differentiation of stem cells. | en_US |
dc.description.sponsorship | Stem Cell and Regenerative Biology | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | The Company of Biologists | en_US |
dc.relation.isversionof | doi:10.1242/dev.085431 | en_US |
dash.license | LAA | |
dc.title | Functional evaluation of ES cell-derived endodermal populations reveals differences between Nodal and Activin A-guided differentiation | en_US |
dc.type | Journal Article | en_US |
dc.date.updated | 2014-02-07T14:58:57Z | |
dc.description.version | Version of Record | en_US |
dc.rights.holder | Chen AE, Borowiak, M, Sherwood R, Kweudjeu A, Melton, DA | |
dc.relation.journal | Development | en_US |
dash.depositing.author | Melton, Douglas A. | |
dc.date.available | 2014-03-07T12:57:30Z | |
dc.identifier.doi | 10.1242/dev.085431 | * |
workflow.legacycomments | In this journal's terms <http://www.biologists.com/copyright_permissions.html#open> they say that articles under the Open option are CC-BY and will be made available immediately ON THEIR WEBSITE. This article, on the website, says "Free" next to the PDF and full text options; other articles, such as doi: 10.1242/dev.101915 do not, and lead me to a pay wall. Therefore, I conclude this item is CC-BY. | en_US |
dash.contributor.affiliated | Sherwood, Richard | |
dash.contributor.affiliated | Melton, Douglas | |