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dc.contributor.authorBellavia, Andreaen_US
dc.contributor.authorUrch, Bruceen_US
dc.contributor.authorSpeck, Maryen_US
dc.contributor.authorBrook, Robert D.en_US
dc.contributor.authorScott, Jeremy A.en_US
dc.contributor.authorAlbetti, Benedettaen_US
dc.contributor.authorBehbod, Behroozen_US
dc.contributor.authorNorth, Michelleen_US
dc.contributor.authorValeri, Lindaen_US
dc.contributor.authorBertazzi, Pier Albertoen_US
dc.contributor.authorSilverman, Francesen_US
dc.contributor.authorGold, Dianeen_US
dc.contributor.authorA. Baccarelli, Andreaen_US
dc.date.accessioned2014-03-10T16:16:21Z
dc.date.issued2013en_US
dc.identifier.citationBellavia, A., B. Urch, M. Speck, R. D. Brook, J. A. Scott, B. Albetti, B. Behbod, et al. 2013. “DNA Hypomethylation, Ambient Particulate Matter, and Increased Blood Pressure: Findings From Controlled Human Exposure Experiments.” Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease 2 (3): e000212. doi:10.1161/JAHA.113.000212. http://dx.doi.org/10.1161/JAHA.113.000212.en
dc.identifier.issn2047-9980en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11877005
dc.description.abstractBackground: Short‐term exposures to fine (<2.5 μm aerodynamic diameter) ambient particulate‐matter (PM) have been related with increased blood pressure (BP) in controlled‐human exposure and community‐based studies. However, whether coarse (2.5 to 10 μm) PM exposure increases BP is uncertain. Recent observational studies have linked PM exposures with blood DNA hypomethylation, an epigenetic alteration that activates inflammatory and vascular responses. No experimental evidence is available to confirm those observational data and demonstrate the relations between PM, hypomethylation, and BP. Methods and Results: We conducted a cross‐over trial of controlled‐human exposure to concentrated ambient particles (CAPs). Fifteen healthy adult participants were exposed for 130 minutes to fine CAPs, coarse CAPs, or HEPA‐filtered medical air (control) in randomized order with ≥2‐week washout. Repetitive‐element (Alu, long interspersed nuclear element‐1 [LINE‐1]) and candidate‐gene (TLR4, IL‐12, IL‐6, iNOS) blood methylation, systolic and diastolic BP were measured pre‐ and postexposure. After adjustment for multiple comparisons, fine CAPs exposure lowered Alu methylation (β‐standardized=−0.74, adjusted‐P=0.03); coarse CAPs exposure lowered TLR4 methylation (β‐standardized=−0.27, adjusted‐P=0.04). Both fine and coarse CAPs determined significantly increased systolic BP (β=2.53 mm Hg, P=0.001; β=1.56 mm Hg, P=0.03, respectively) and nonsignificantly increased diastolic BP (β=0.98 mm Hg, P=0.12; β=0.82 mm Hg, P=0.11, respectively). Decreased Alu and TLR4 methylation was associated with higher postexposure DBP (β‐standardized=0.41, P=0.04; and β‐standardized=0.84, P=0.02; respectively). Decreased TLR4 methylation was associated with higher postexposure SBP (β‐standardized=1.45, P=0.01). Conclusions: Our findings provide novel evidence of effects of coarse PM on BP and confirm effects of fine PM. Our results provide the first experimental evidence of PM‐induced DNA hypomethylation and its correlation to BP.en
dc.language.isoen_USen
dc.publisherBlackwell Publishing Ltden
dc.relation.isversionofdoi:10.1161/JAHA.113.000212en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698788/pdf/en
dash.licenseLAAen_US
dc.subjectGeneticsen
dc.subjectair pollutionen
dc.subjectblood pressureen
dc.subjectDNA methylationen
dc.subjectepigeneticsen
dc.subjectmediationen
dc.titleDNA Hypomethylation, Ambient Particulate Matter, and Increased Blood Pressure: Findings From Controlled Human Exposure Experimentsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalJournal of the American Heart Association: Cardiovascular and Cerebrovascular Diseaseen
dash.depositing.authorValeri, Lindaen_US
dc.date.available2014-03-10T16:16:21Z
dc.identifier.doi10.1161/JAHA.113.000212*
dash.authorsorderedfalse
dash.contributor.affiliatedValeri, Linda
dash.contributor.affiliatedGold, Diane


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