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dc.contributor.authorKhatri, Madhuen_US
dc.contributor.authorBello, Dhimiteren_US
dc.contributor.authorPal, Anoop Ken_US
dc.contributor.authorCohen, Joel Men_US
dc.contributor.authorWoskie, Susanen_US
dc.contributor.authorGassert, Thomasen_US
dc.contributor.authorLan, Jiaqien_US
dc.contributor.authorGu, April Zen_US
dc.contributor.authorDemokritou, Philipen_US
dc.contributor.authorGaines, Peteren_US
dc.date.accessioned2014-03-10T16:16:34Z
dc.date.issued2013en_US
dc.identifier.citationKhatri, Madhu, Dhimiter Bello, Anoop K Pal, Joel M Cohen, Susan Woskie, Thomas Gassert, Jiaqi Lan, April Z Gu, Philip Demokritou, and Peter Gaines. 2013. “Evaluation of cytotoxic, genotoxic and inflammatory responses of nanoparticles from photocopiers in three human cell lines.” Particle and Fibre Toxicology 10 (1): 42. doi:10.1186/1743-8977-10-42. http://dx.doi.org/10.1186/1743-8977-10-42.en
dc.identifier.issn1743-8977en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11877022
dc.description.abstractBackground: Photocopiers emit nanoparticles with complex chemical composition. Short-term exposures to modest nanoparticle concentrations triggered upper airway inflammation and oxidative stress in healthy human volunteers in a recent study. To further understand the toxicological properties of copier-emitted nanoparticles, we studied in-vitro their ability to induce cytotoxicity, pro-inflammatory cytokine release, DNA damage, and apoptosis in relevant human cell lines. Methods: Three cell types were used: THP-1, primary human nasal- and small airway epithelial cells. Following collection in a large volume photocopy center, nanoparticles were extracted, dispersed and characterized in the cell culture medium. Cells were doped at 30, 100 and 300 μg/mL administered doses for up to 24 hrs. Estimated dose delivered to cells, was ~10% and 22% of the administered dose at 6 and 24 hrs, respectively. Gene expression analysis of key biomarkers was performed using real time quantitative PCR (RT-qPCR) in THP-1 cells at 5 μg nanoparticles/mL for 6-hr exposure for confirmation purposes. Results: Multiple cytokines, GM-CSF, IL-1β, IL-6, IL-8, IFNγ, MCP-1, TNF-α and VEGF, were significantly elevated in THP-1 cells in a dose-dependent manner. Gene expression analysis confirmed up-regulation of the TNF-α gene in THP-1 cells, consistent with cytokine findings. In both primary epithelial cells, cytokines IL-8, VEGF, EGF, IL-1α, TNF-α, IL-6 and GM-CSF were significantly elevated. Apoptosis was induced in all cell lines in a dose-dependent manner, consistent with the significant up-regulation of key apoptosis-regulating genes P53 and Casp8 in THP-1 cells. No significant DNA damage was found at any concentration with the comet assay. Up-regulation of key DNA damage and repair genes, Ku70 and Rad51, were also observed in THP-1 cells, albeit not statistically significant. Significant up-regulation of the key gene HO1 for oxidative stress, implicates oxidative stress induced by nanoparticles. Conclusions: Copier-emitted nanoparticles induced the release of pro-inflammatory cytokines, apoptosis and modest cytotoxicity but no DNA damage in all three-human cell lines. Taken together with gene expression data in THP-1 cells, we conclude that these nanoparticles are directly responsible for inflammation observed in human volunteers. Further toxicological evaluations of these nanoparticles, including across different toner formulations, are warranted.en
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.relation.isversionofdoi:10.1186/1743-8977-10-42en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766213/pdf/en
dash.licenseLAAen_US
dc.subjectEngineered nanoparticlesen
dc.subjectPhotocopieren
dc.subjectPrinteren
dc.subjectToneren
dc.subjectInflammationen
dc.subjectApoptosisen
dc.subjectDNA damageen
dc.titleEvaluation of cytotoxic, genotoxic and inflammatory responses of nanoparticles from photocopiers in three human cell linesen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalParticle and Fibre Toxicologyen
dash.depositing.authorGassert, Thomasen_US
dc.date.available2014-03-10T16:16:34Z
dc.identifier.doi10.1186/1743-8977-10-42*
dash.contributor.affiliatedGassert, Thomas
dash.contributor.affiliatedDemokritou, Philip


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