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dc.contributor.authorBeaudoin, Mélissaen_US
dc.contributor.authorGoyette, Philippeen_US
dc.contributor.authorBoucher, Gabrielleen_US
dc.contributor.authorLo, Ken Sinen_US
dc.contributor.authorRivas, Manuel A.en_US
dc.contributor.authorStevens, Christineen_US
dc.contributor.authorAlikashani, Azadehen_US
dc.contributor.authorLadouceur, Martinen_US
dc.contributor.authorEllinghaus, Daviden_US
dc.contributor.authorTörkvist, Leifen_US
dc.contributor.authorGoel, Gautamen_US
dc.contributor.authorLagacé, Carolineen_US
dc.contributor.authorAnnese, Vitoen_US
dc.contributor.authorBitton, Alainen_US
dc.contributor.authorBegun, Jakoben_US
dc.contributor.authorBrant, Steve R.en_US
dc.contributor.authorBresso, Francescaen_US
dc.contributor.authorCho, Judy H.en_US
dc.contributor.authorDuerr, Richard H.en_US
dc.contributor.authorHalfvarson, Jonasen_US
dc.contributor.authorMcGovern, Dermot P. B.en_US
dc.contributor.authorRadford-Smith, Grahamen_US
dc.contributor.authorSchreiber, Stefanen_US
dc.contributor.authorSchumm, Philip L.en_US
dc.contributor.authorSharma, Yashodaen_US
dc.contributor.authorSilverberg, Mark S.en_US
dc.contributor.authorWeersma, Rinse K.en_US
dc.contributor.authorD'Amato, Mauroen_US
dc.contributor.authorVermeire, Severineen_US
dc.contributor.authorFranke, Andreen_US
dc.contributor.authorLettre, Guillaumeen_US
dc.contributor.authorXavier, Ramnik J.en_US
dc.contributor.authorDaly, Mark J.en_US
dc.contributor.authorRioux, John D.en_US
dc.date.accessioned2014-03-10T16:17:14Z
dc.date.issued2013en_US
dc.identifier.citationBeaudoin, M., P. Goyette, G. Boucher, K. S. Lo, M. A. Rivas, C. Stevens, A. Alikashani, et al. 2013. “Deep Resequencing of GWAS Loci Identifies Rare Variants in CARD9, IL23R and RNF186 That Are Associated with Ulcerative Colitis.” PLoS Genetics 9 (9): e1003723. doi:10.1371/journal.pgen.1003723. http://dx.doi.org/10.1371/journal.pgen.1003723.en
dc.identifier.issn1553-7390en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11877070
dc.description.abstractGenome-wide association studies and follow-up meta-analyses in Crohn's disease (CD) and ulcerative colitis (UC) have recently identified 163 disease-associated loci that meet genome-wide significance for these two inflammatory bowel diseases (IBD). These discoveries have already had a tremendous impact on our understanding of the genetic architecture of these diseases and have directed functional studies that have revealed some of the biological functions that are important to IBD (e.g. autophagy). Nonetheless, these loci can only explain a small proportion of disease variance (∼14% in CD and 7.5% in UC), suggesting that not only are additional loci to be found but that the known loci may contain high effect rare risk variants that have gone undetected by GWAS. To test this, we have used a targeted sequencing approach in 200 UC cases and 150 healthy controls (HC), all of French Canadian descent, to study 55 genes in regions associated with UC. We performed follow-up genotyping of 42 rare non-synonymous variants in independent case-control cohorts (totaling 14,435 UC cases and 20,204 HC). Our results confirmed significant association to rare non-synonymous coding variants in both IL23R and CARD9, previously identified from sequencing of CD loci, as well as identified a novel association in RNF186. With the exception of CARD9 (OR = 0.39), the rare non-synonymous variants identified were of moderate effect (OR = 1.49 for RNF186 and OR = 0.79 for IL23R). RNF186 encodes a protein with a RING domain having predicted E3 ubiquitin-protein ligase activity and two transmembrane domains. Importantly, the disease-coding variant is located in the ubiquitin ligase domain. Finally, our results suggest that rare variants in genes identified by genome-wide association in UC are unlikely to contribute significantly to the overall variance for the disease. Rather, these are expected to help focus functional studies of the corresponding disease loci.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pgen.1003723en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3772057/pdf/en
dash.licenseLAAen_US
dc.titleDeep Resequencing of GWAS Loci Identifies Rare Variants in CARD9, IL23R and RNF186 That Are Associated with Ulcerative Colitisen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS Geneticsen
dash.depositing.authorGoel, Gautamen_US
dc.date.available2014-03-10T16:17:14Z
dc.identifier.doi10.1371/journal.pgen.1003723*
dash.authorsorderedfalse
dash.contributor.affiliatedGoel, Gautam
dash.contributor.affiliatedDaly, Mark
dash.contributor.affiliatedXavier, Ramnik


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