Segregation of molecules at cell division reveals native protein localization

DSpace/Manakin Repository

Segregation of molecules at cell division reveals native protein localization

Show simple item record

dc.contributor.author Landgraf, Dirk en_US
dc.contributor.author Okumus, Burak en_US
dc.contributor.author Chien, Peter en_US
dc.contributor.author Baker, Tania A. en_US
dc.contributor.author Paulsson, Johan en_US
dc.date.accessioned 2014-03-10T16:17:22Z
dc.date.issued 2013 en_US
dc.identifier.citation Landgraf, Dirk, Burak Okumus, Peter Chien, Tania A. Baker, and Johan Paulsson. 2013. “Segregation of molecules at cell division reveals native protein localization.” Nature methods 9 (5): 480-482. doi:10.1038/nmeth.1955. http://dx.doi.org/10.1038/nmeth.1955. en
dc.identifier.issn 1548-7091 en
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:11877087
dc.description.abstract We introduce a non-intrusive method exploiting post-division single-cell variability to validate protein localization. The results show that Clp proteases, widely reported to form biologically relevant foci, are in fact uniformly distributed inside Escherichia coli cells, and that many commonly used fluorescent proteins (FPs) cause severe mislocalization when fused to homo-oligomers. Re-tagging five other reportedly foci-forming proteins with the most monomeric FP tested suggests the foci were caused by the FPs. en
dc.language.iso en_US en
dc.relation.isversionof doi:10.1038/nmeth.1955 en
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779060/pdf/ en
dash.license LAA en_US
dc.title Segregation of molecules at cell division reveals native protein localization en
dc.type Journal Article en_US
dc.description.version Version of Record en
dc.relation.journal Nature methods en
dash.depositing.author Okumus, Burak en_US
dc.date.available 2014-03-10T16:17:22Z

Files in this item

Files Size Format View
3779060.pdf 1.133Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

 
 

Search DASH


Advanced Search
 
 

Submitters