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dc.contributor.authorLandgraf, Dirken_US
dc.contributor.authorOkumus, Buraken_US
dc.contributor.authorChien, Peteren_US
dc.contributor.authorBaker, Tania A.en_US
dc.contributor.authorPaulsson, Johanen_US
dc.date.accessioned2014-03-10T16:17:22Z
dc.date.issued2013en_US
dc.identifier.citationLandgraf, Dirk, Burak Okumus, Peter Chien, Tania A. Baker, and Johan Paulsson. 2013. “Segregation of molecules at cell division reveals native protein localization.” Nature methods 9 (5): 480-482. doi:10.1038/nmeth.1955. http://dx.doi.org/10.1038/nmeth.1955.en
dc.identifier.issn1548-7091en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11877087
dc.description.abstractWe introduce a non-intrusive method exploiting post-division single-cell variability to validate protein localization. The results show that Clp proteases, widely reported to form biologically relevant foci, are in fact uniformly distributed inside Escherichia coli cells, and that many commonly used fluorescent proteins (FPs) cause severe mislocalization when fused to homo-oligomers. Re-tagging five other reportedly foci-forming proteins with the most monomeric FP tested suggests the foci were caused by the FPs.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nmeth.1955en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779060/pdf/en
dash.licenseLAAen_US
dc.titleSegregation of molecules at cell division reveals native protein localizationen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature methodsen
dash.depositing.authorOkumus, Buraken_US
dc.date.available2014-03-10T16:17:22Z
dc.identifier.doi10.1038/nmeth.1955*
dash.contributor.affiliatedOkumus, Burak
dash.contributor.affiliatedPaulsson, Johan


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