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dc.contributor.authorWhiteman, Noah K.
dc.contributor.authorGloss, Andrew D.
dc.contributor.authorSackton, Timothy
dc.contributor.authorGroen, Simon C.
dc.contributor.authorHumphrey, Parris T.
dc.contributor.authorLapoint, Richard T.
dc.contributor.authorSønderby, Ida E.
dc.contributor.authorHalkier, Barbara A.
dc.contributor.authorKocks, Christine
dc.contributor.authorAusubel, Frederick M.
dc.contributor.authorPierce, Naomi Ellen
dc.date.accessioned2014-03-10T17:43:08Z
dc.date.issued2012
dc.identifier.citationWhiteman, Noah K., Andrew D. Gloss, Timothy B. Sackton, Simon C. Groen, Parris T. Humphrey, Richard T. Lapoint, Ida E. Sønderby, et al. 2012. Genes Involved in the Evolution of Herbivory by a Leaf-Mining, Drosophilid Fly. Genome Biology and Evolution 4, no. 9: 788–804.en_US
dc.identifier.issn1759-6653en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11878759
dc.description.abstractHerbivorous insects are among the most successful radiations of life. However, we know little about the processes underpinning the evolution of herbivory. We examined the evolution of herbivory in the fly, Scaptomyza flava, whose larvae are leaf miners on species of Brassicaceae, including the widely studied reference plant, Arabidopsis thaliana (Arabidopsis). Scaptomyza flava is phylogenetically nested within the paraphyletic genus Drosophila, and the whole genome sequences available for 12 species of Drosophila facilitated phylogenetic analysis and assembly of a transcriptome for S. flava. A time-calibrated phylogeny indicated that leaf mining in Scaptomyza evolved between 6 and 16 million years ago. Feeding assays showed that biosynthesis of glucosinolates, the major class of antiherbivore chemical defense compounds in mustard leaves, was upregulated by S. flava larval feeding. The presence of glucosinolates in wild-type (WT) Arabidopsis plants reduced S. flava larval weight gain and increased egg–adult development time relative to flies reared in glucosinolate knockout (GKO) plants. An analysis of gene expression differences in 5-day-old larvae reared on WT versus GKO plants showed a total of 341 transcripts that were differentially regulated by glucosinolate uptake in larval S. flava. Of these, approximately a third corresponded to homologs of Drosophila melanogaster genes associated with starvation, dietary toxin-, heat-, oxidation-, and aging-related stress. The upregulated transcripts exhibited elevated rates of protein evolution compared with unregulated transcripts. The remaining differentially regulated transcripts also contained a higher proportion of novel genes than the unregulated transcripts. Thus, the transition to herbivory in Scaptomyza appears to be coupled with the evolution of novel genes and the co-option of conserved stress-related genes.en_US
dc.description.sponsorshipOrganismic and Evolutionary Biologyen_US
dc.language.isoen_USen_US
dc.publisherOxford University Press (OUP)en_US
dc.relation.isversionofdoi:10.1093/gbe/evs063en_US
dc.relation.hasversionhttp://www.oeb.harvard.edu/faculty/pierce/publications/pdfs/2012_Whiteman_et_al_GBE.pdfen_US
dash.licenseLAA
dc.subjectArabidopsisen_US
dc.subjectDrosophilaen_US
dc.subjectglucosinolatesen_US
dc.subjectherbivoryen_US
dc.subjecthost specializationen_US
dc.subjecttranscriptomeen_US
dc.titleGenes Involved in the Evolution of Herbivory by a Leaf-Mining, Drosophilid Flyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalGenome Biology and Evolutionen_US
dash.depositing.authorPierce, Naomi Ellen
dc.date.available2014-03-10T17:43:08Z
dash.affiliation.otherDepartment of Pediatrics, Harvard Medical Schoolen_US
dash.affiliation.otherDepartment of Genetics, Harvard Medical Schoolen_US
dc.identifier.doi10.1093/gbe/evs063*
workflow.legacycommentsCan post pub per Sherpa. http://www.sherpa.ac.uk/romeo/issn/1759-6653/ FAR 2013en_US
dash.authorsorderedfalse
dash.contributor.affiliatedKocks, Christine
dash.contributor.affiliatedAusubel, Frederick
dash.contributor.affiliatedSackton, Timothy
dash.contributor.affiliatedPierce, Naomi


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