A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
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Author
Zhang, Chen
Jang, Sunyoung
Shimizu, Koichi
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https://doi.org/10.1155/2013/373569Metadata
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Zhang, Chen, Sunyoung Jang, Ovid C. Amadi, Koichi Shimizu, Richard T. Lee, and Richard N. Mitchell. 2013. “A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device.” BioMed Research International 2013 (1): 373569. doi:10.1155/2013/373569. http://dx.doi.org/10.1155/2013/373569.Abstract
Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787577/pdf/Terms of Use
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