Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells

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Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells

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Title: Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells
Author: Li, Wei; Lee, Samuel; Ma, Minglin; Kim, Soo Min; Guye, Patrick; Pancoast, James R.; Anderson, Daniel G.; Weiss, Ron; Lee, Richard T.; Hammond, Paula T.

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Citation: Li, Wei, Samuel Lee, Minglin Ma, Soo Min Kim, Patrick Guye, James R. Pancoast, Daniel G. Anderson, Ron Weiss, Richard T. Lee, and Paula T. Hammond. 2013. “Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells.” Scientific Reports 3 (1): 2863. doi:10.1038/srep02863. http://dx.doi.org/10.1038/srep02863.
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Abstract: Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary β-cells in direct contact with ‘synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of β-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced β-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types.
Published Version: doi:10.1038/srep02863
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790197/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11878825
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