Show simple item record

dc.contributor.authorSander, Jeffry D.en_US
dc.contributor.authorRamirez, Cherie L.en_US
dc.contributor.authorLinder, Samantha J.en_US
dc.contributor.authorPattanayak, Vikramen_US
dc.contributor.authorShoresh, Noamen_US
dc.contributor.authorKu, Manchingen_US
dc.contributor.authorFoden, Jennifer A.en_US
dc.contributor.authorReyon, Deepaken_US
dc.contributor.authorBernstein, Bradley E.en_US
dc.contributor.authorLiu, David R.en_US
dc.contributor.authorJoung, J. Keithen_US
dc.date.accessioned2014-03-10T20:33:23Z
dc.date.issued2013en_US
dc.identifier.citationSander, J. D., C. L. Ramirez, S. J. Linder, V. Pattanayak, N. Shoresh, M. Ku, J. A. Foden, et al. 2013. “In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites.” Nucleic Acids Research 41 (19): e181. doi:10.1093/nar/gkt716. http://dx.doi.org/10.1093/nar/gkt716.en
dc.identifier.issn0305-1048en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11878851
dc.description.abstractGene-editing nucleases enable targeted modification of DNA sequences in living cells, thereby facilitating efficient knockout and precise editing of endogenous loci. Engineered nucleases also have the potential to introduce mutations at off-target sites of action. Such unintended alterations can confound interpretation of experiments and can have implications for development of therapeutic applications. Recently, two improved methods for identifying the off-target effects of zinc finger nucleases (ZFNs) were described–one using an in vitro cleavage site selection method and the other exploiting the insertion of integration-defective lentiviruses into nuclease-induced double-stranded DNA breaks. However, application of these two methods to a ZFN pair targeted to the human CCR5 gene led to identification of largely non-overlapping off-target sites, raising the possibility that additional off-target sites might exist. Here, we show that in silico abstraction of ZFN cleavage profiles obtained from in vitro cleavage site selections can greatly enhance the ability to identify potential off-target sites in human cells. Our improved method should enable more comprehensive profiling of ZFN specificities.en
dc.language.isoen_USen
dc.publisherOxford University Pressen
dc.relation.isversionofdoi:10.1093/nar/gkt716en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799455/pdf/en
dash.licenseLAAen_US
dc.titleIn silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sitesen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNucleic Acids Researchen
dash.depositing.authorSander, Jeffry D.en_US
dc.date.available2014-03-10T20:33:23Z
dc.identifier.doi10.1093/nar/gkt716*
dash.authorsorderedfalse
dash.contributor.affiliatedLinder, Sam
dash.contributor.affiliatedPattanayak, Vikram
dash.contributor.affiliatedRamirez, Cherie Lynn
dash.contributor.affiliatedSander, Jeffry D.
dash.contributor.affiliatedReyon, Deepak
dash.contributor.affiliatedLiu, David
dash.contributor.affiliatedBernstein, Bradley


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record