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dc.contributor.authorMirkin, Ekaterina V.en_US
dc.contributor.authorChang, Frederick S.en_US
dc.contributor.authorKleckner, Nancyen_US
dc.date.accessioned2014-03-10T20:33:34Z
dc.date.issued2013en_US
dc.identifier.citationMirkin, Ekaterina V., Frederick S. Chang, and Nancy Kleckner. 2013. “Dynamic Trans Interactions in Yeast Chromosomes.” PLoS ONE 8 (9): e75895. doi:10.1371/journal.pone.0075895. http://dx.doi.org/10.1371/journal.pone.0075895.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11878866
dc.description.abstractThree-dimensional organization of the genome is important for regulation of gene expression and maintenance of genomic stability. It also defines, and is defined by, contacts between different chromosomal loci. Interactions between loci positioned on different chromosomes, i.e. “trans” interactions are one type of such contacts. Here, we describe a case of inducible trans interaction in chromosomes of the budding yeast S. cerevisiae. Special DNA sequences, inserted in two ectopic chromosomal loci positioned in trans, pair with one another in an inducible manner. The spatial proximity diagnostic of pairing is observable by both chromosome capture analysis (3C) and epifluorescence microscopy in whole cells. Protein synthesis de novo appears to be required for this process. The three-dimensional organization of the yeast nucleus imposes a constraint on such pairing, presumably by dictating the probability with which the two sequences collide with one another.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0075895en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786970/pdf/en
dash.licenseLAAen_US
dc.titleDynamic Trans Interactions in Yeast Chromosomesen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorMirkin, Ekaterina V.en_US
dc.date.available2014-03-10T20:33:34Z
dc.identifier.doi10.1371/journal.pone.0075895*
dash.contributor.affiliatedMirkin, Ekaterina V.
dash.contributor.affiliatedChang, Frederick S.
dash.contributor.affiliatedKleckner, Nancy


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