A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells
Maher, Christopher A.
Daub, Carsten O.
Caballero, Otavia L.
Lawlor, Elizabeth R.
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CitationSompallae, R., O. Hofmann, C. A. Maher, C. Gedye, A. Behren, M. Vitezic, C. O. Daub, et al. 2013. “A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells.” Frontiers in Genetics 4 (1): 209. doi:10.3389/fgene.2013.00209. http://dx.doi.org/10.3389/fgene.2013.00209.
AbstractPROM1 is the gene encoding prominin-1 or CD133, an important cell surface marker for the isolation of both normal and cancer stem cells. PROM1 transcripts initiate at a range of transcription start sites (TSS) associated with distinct tissue and cancer expression profiles. Using high resolution Cap Analysis of Gene Expression (CAGE) sequencing we characterize TSS utilization across a broad range of normal and developmental tissues. We identify a novel proximal promoter (P6) within CD133+ melanoma cell lines and stem cells. Additional exon array sampling finds P6 to be active in populations enriched for mesenchyme, neural stem cells and within CD133+ enriched Ewing sarcomas. The P6 promoter is enriched with respect to previously characterized PROM1 promoters for a HMGI/Y (HMGA1) family transcription factor binding site motif and exhibits different epigenetic modifications relative to the canonical promoter region of PROM1.
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