NEW TOOLS FOR THE BRAINBOW TOOLBOX

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NEW TOOLS FOR THE BRAINBOW TOOLBOX

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Title: NEW TOOLS FOR THE BRAINBOW TOOLBOX
Author: Cai, Dawen; Cohen, Kimberly B.; Luo, Tuanlian; Lichtman, Jeff W.; Sanes, Joshua R.

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Citation: Cai, Dawen, Kimberly B. Cohen, Tuanlian Luo, Jeff W. Lichtman, and Joshua R. Sanes. 2013. “NEW TOOLS FOR THE BRAINBOW TOOLBOX.” Nature methods 10 (6): 540-547. doi:10.1038/nmeth.2450. http://dx.doi.org/10.1038/nmeth.2450.
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Abstract: In a recently introduced transgenic multicolor labeling strategy called “Brainbow,” Cre-lox recombination is used to create a stochastic choice of expression among fluorescent proteins (XFPs), resulting in the indelible marking of mouse neurons with multiple, distinct colors. This method has been adapted to non-neuronal cells in mice and to neurons in fish and flies, but has yet to realize its full potential in the mouse brain. Here, we present several new lines of mice that overcome limitations of the initial lines and an adaptation of the method for use in adeno-associated viral (AAV) vectors. We also provide technical advice about how best to image Brainbow transgenes.
Published Version: doi:10.1038/nmeth.2450
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713494/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879049
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