Live single cell functional phenotyping in droplet nano-liter reactors

DSpace/Manakin Repository

Live single cell functional phenotyping in droplet nano-liter reactors

Citable link to this page

 

 
Title: Live single cell functional phenotyping in droplet nano-liter reactors
Author: Konry, Tania; Golberg, Alexander; Yarmush, Martin

Note: Order does not necessarily reflect citation order of authors.

Citation: Konry, Tania, Alexander Golberg, and Martin Yarmush. 2013. “Live single cell functional phenotyping in droplet nano-liter reactors.” Scientific Reports 3 (1): 3179. doi:10.1038/srep03179. http://dx.doi.org/10.1038/srep03179.
Full Text & Related Files:
Abstract: While single cell heterogeneity is present in all biological systems, most studies cannot address it due to technical limitations. Here we describe a nano-liter droplet microfluidic-based approach for stimulation and monitoring of surfaceand secreted markers of live single immune dendritic cells (DCs) as well as monitoring the live T cell/DC interaction. This nano-liter in vivo simulating microenvironment allows delivering various stimuli reagents to each cell and appropriate gas exchanges which are necessary to ensure functionality and viability of encapsulated cells. Labeling bioassay and microsphere sensors were integrated into nano-liter reaction volume of the droplet to monitor live single cell surface markers and secretion analysis in the time-dependent fashion. Thus live cell stimulation, secretion and surface monitoring can be obtained simultaneously in distinct microenvironment, which previously was possible using complicated and multi-step in vitro and in vivo live-cell microscopy, together with immunological studies of the outcome secretion of cellular function.
Published Version: doi:10.1038/srep03179
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822379/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879074
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters