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dc.contributor.authorLee, Jonghyeoben_US
dc.contributor.authorSugiyama, Takuyaen_US
dc.contributor.authorLiu, Yinghuaen_US
dc.contributor.authorWang, Jingen_US
dc.contributor.authorGu, Xueyingen_US
dc.contributor.authorLei, Jien_US
dc.contributor.authorMarkmann, James Fen_US
dc.contributor.authorMiyazaki, Satsukien_US
dc.contributor.authorMiyazaki, Jun-ichien_US
dc.contributor.authorSzot, Gregory Len_US
dc.contributor.authorBottino, Ritaen_US
dc.contributor.authorKim, Seung Ken_US
dc.date.accessioned2014-03-11T02:49:03Z
dc.date.issued2013en_US
dc.identifier.citationLee, J., T. Sugiyama, Y. Liu, J. Wang, X. Gu, J. Lei, J. F. Markmann, et al. 2013. “Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells.” eLife 2 (1): e00940. doi:10.7554/eLife.00940. http://dx.doi.org/10.7554/eLife.00940.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879086
dc.description.abstractPancreatic islet β-cell insufficiency underlies pathogenesis of diabetes mellitus; thus, functional β-cell replacement from renewable sources is the focus of intensive worldwide effort. However, in vitro production of progeny that secrete insulin in response to physiological cues from primary human cells has proven elusive. Here we describe fractionation, expansion and conversion of primary adult human pancreatic ductal cells into progeny resembling native β-cells. FACS-sorted adult human ductal cells clonally expanded as spheres in culture, while retaining ductal characteristics. Expression of the cardinal islet developmental regulators Neurog3, MafA, Pdx1 and Pax6 converted exocrine duct cells into endocrine progeny with hallmark β-cell properties, including the ability to synthesize, process and store insulin, and secrete it in response to glucose or other depolarizing stimuli. These studies provide evidence that genetic reprogramming of expandable human pancreatic cells with defined factors may serve as a general strategy for islet replacement in diabetes. DOI: http://dx.doi.org/10.7554/eLife.00940.001en
dc.language.isoen_USen
dc.publishereLife Sciences Publications, Ltden
dc.relation.isversionofdoi:10.7554/eLife.00940en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826580/pdf/en
dash.licenseLAAen_US
dc.subjectdiabetesen
dc.subjectisleten
dc.subjectpancreasen
dc.subjectbeta-cellsen
dc.subjectinsulinen
dc.subjectconversionen
dc.subjectHumanen
dc.titleExpansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cellsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journaleLifeen
dash.depositing.authorLei, Jien_US
dc.date.available2014-03-11T02:49:03Z
dc.identifier.doi10.7554/eLife.00940*
dash.authorsorderedfalse
dash.contributor.affiliatedLei, Ji
dash.contributor.affiliatedMarkmann, James


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