Bioorthogonal Small Molecule Imaging Agents Allow Single-Cell Imaging of MET
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CitationKim, Eunha, Katherine S. Yang, and Ralph Weissleder. 2013. “Bioorthogonal Small Molecule Imaging Agents Allow Single-Cell Imaging of MET.” PLoS ONE 8 (11): e81275. doi:10.1371/journal.pone.0081275. http://dx.doi.org/10.1371/journal.pone.0081275.
AbstractThe hepatocyte growth factor receptor (MET) is a receptor tyrosine kinase (RTK) that has emerged as an important cancer target. Consequently, a number of different inhibitors varying in specificity are currently in clinical development. However, to date, it has been difficult to visualize MET expression, intracellular drug distribution and small molecule MET inhibition. Using a bioorthogonal approach, we have developed two companion imaging drugs based on both mono- and polypharmacological MET inhibitors. We show exquisite drug and target co-localization that can be visualized at single-cell resolution. The developed agents may be useful chemical biology tools to investigate single-cell pharmacokinetics and pharmacodynamics of MET inhibitors.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879093
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