Barcoding cells using cell-surface programmable DNA-binding domains
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CitationMali, Prashant, John Aach, Jehyuk Lee, Daniel Levner, Lisa Nip, and George M. Church. 2013. “Barcoding cells using cell-surface programmable DNA-binding domains.” Nature methods 10 (5): 403-406. doi:10.1038/nmeth.2407. http://dx.doi.org/10.1038/nmeth.2407.
AbstractWe develop here a novel approach to barcode large numbers of cells through cell-surface expression of programmable zinc-finger DNA-binding domains (sZFs). We show sZFs enable double-stranded DNA to sequence-specifically label living cells, and also develop a sequential tagging approach to in situ image >3 cell types using just 3 fluorophores. Finally we demonstrate their broad versatility through ability to serve as surrogate reporters and facilitate selective cell capture and targeting.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879101
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