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dc.contributor.authorRayamajhi, Nabinen_US
dc.contributor.authorKim, Seul-Kien_US
dc.contributor.authorGo, Hiroeen_US
dc.contributor.authorJoe, Yeonsooen_US
dc.contributor.authorCallaway, Zaken_US
dc.contributor.authorKang, Jae-Guen_US
dc.contributor.authorRyter, Stefan W.en_US
dc.contributor.authorChung, Hun Taegen_US
dc.date.accessioned2014-03-11T02:49:19Z
dc.date.issued2013en_US
dc.identifier.citationRayamajhi, Nabin, Seul-Ki Kim, Hiroe Go, Yeonsoo Joe, Zak Callaway, Jae-Gu Kang, Stefan W. Ryter, and Hun Taeg Chung. 2013. “Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells.” Oxidative Medicine and Cellular Longevity 2013 (1): 154279. doi:10.1155/2013/154279. http://dx.doi.org/10.1155/2013/154279.en
dc.identifier.issn1942-0900en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879116
dc.description.abstractThe regeneration of mitochondria by regulated biogenesis plays an important homeostatic role in cells and tissues and furthermore may provide an adaptive mechanism in certain diseases such as sepsis. The heme oxygenase (HO-1)/carbon monoxide (CO) system is an inducible cytoprotective mechanism in mammalian cells. Natural antioxidants can provide therapeutic benefit, in part, by inducing the HO-1/CO system. This study focused on the mechanism by which the natural antioxidant quercetin can induce mitochondrial biogenesis in HepG2 cells. We found that quercetin treatment induced expression of mitochondrial biogenesis activators (PGC-1α, NRF-1, TFAM), mitochondrial DNA (mtDNA), and proteins (COX IV) in HepG2 cells. The HO inhibitor SnPP and the CO scavenger hemoglobin reversed the effects of quercetin on mitochondrial biogenesis in HepG2 cells. The stimulatory effects of quercetin on mitochondrial biogenesis could be recapitulated in vivo in liver tissue and antagonized by SnPP. Finally, quercetin conferred an anti-inflammatory effect in the liver of mice treated with LPS and prevented impairment of mitochondrial biogenesis by LPS in vivo. These salutary effects of quercetin in vivo were also antagonized by SnPP. Thus, our results suggest that quercetin enhances mitochondrial biogenesis mainly via the HO-1/CO system in vitro and in vivo. The beneficial effects of quercetin may provide a therapeutic basis in inflammatory diseases and sepsis.en
dc.language.isoen_USen
dc.publisherHindawi Publishing Corporationen
dc.relation.isversionofdoi:10.1155/2013/154279en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833383/pdf/en
dash.licenseLAAen_US
dc.titleQuercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cellsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalOxidative Medicine and Cellular Longevityen
dash.depositing.authorRyter, Stefan W.en_US
dc.date.available2014-03-11T02:49:19Z
dc.identifier.doi10.1155/2013/154279*
dash.contributor.affiliatedRyter, Stefan W.


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