Show simple item record

dc.contributor.authorAkers, Johnny C.en_US
dc.contributor.authorRamakrishnan, Valyaen_US
dc.contributor.authorKim, Ryanen_US
dc.contributor.authorSkog, Johanen_US
dc.contributor.authorNakano, Ichiroen_US
dc.contributor.authorPingle, Sandeepen_US
dc.contributor.authorKalinina, Juliyaen_US
dc.contributor.authorHua, Weien_US
dc.contributor.authorKesari, Santoshen_US
dc.contributor.authorMao, Yingen_US
dc.contributor.authorBreakefield, Xandra O.en_US
dc.contributor.authorHochberg, Fred H.en_US
dc.contributor.authorVan Meir, Erwin G.en_US
dc.contributor.authorCarter, Bob S.en_US
dc.contributor.authorChen, Clark C.en_US
dc.date.accessioned2014-03-11T02:49:38Z
dc.date.issued2013en_US
dc.identifier.citationAkers, J. C., V. Ramakrishnan, R. Kim, J. Skog, I. Nakano, S. Pingle, J. Kalinina, et al. 2013. “miR-21 in the Extracellular Vesicles (EVs) of Cerebrospinal Fluid (CSF): A Platform for Glioblastoma Biomarker Development.” PLoS ONE 8 (10): e78115. doi:10.1371/journal.pone.0078115. http://dx.doi.org/10.1371/journal.pone.0078115.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879153
dc.description.abstractGlioblastoma cells secrete extra-cellular vesicles (EVs) containing microRNAs (miRNAs). Analysis of these EV miRNAs in the bio-fluids of afflicted patients represents a potential platform for biomarker development. However, the analytic algorithm for quantitative assessment of EV miRNA remains under-developed. Here, we demonstrate that the reference transcripts commonly used for quantitative PCR (including GAPDH, 18S rRNA, and hsa-miR-103) were unreliable for assessing EV miRNA. In this context, we quantitated EV miRNA in absolute terms and normalized this value to the input EV number. Using this method, we examined the abundance of miR-21, a highly over-expressed miRNA in glioblastomas, in EVs. In a panel of glioblastoma cell lines, the cellular levels of miR-21 correlated with EV miR-21 levels (p<0.05), suggesting that glioblastoma cells actively secrete EVs containing miR-21. Consistent with this hypothesis, the CSF EV miR-21 levels of glioblastoma patients (n=13) were, on average, ten-fold higher than levels in EVs isolated from the CSF of non-oncologic patients (n=13, p<0.001). Notably, none of the glioblastoma CSF harbored EV miR-21 level below 0.25 copies per EV in this cohort. Using this cut-off value, we were able to prospectively distinguish CSF derived from glioblastoma and non-oncologic patients in an independent cohort of twenty-nine patients (Sensitivity=87%; Specificity=93%; AUC=0.91, p<0.01). Our results suggest that CSF EV miRNA analysis of miR-21 may serve as a platform for glioblastoma biomarker development.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0078115en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804457/pdf/en
dash.licenseLAAen_US
dc.titlemiR-21 in the Extracellular Vesicles (EVs) of Cerebrospinal Fluid (CSF): A Platform for Glioblastoma Biomarker Developmenten
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorBreakefield, Xandra O.en_US
dc.date.available2014-03-11T02:49:38Z
dc.identifier.doi10.1371/journal.pone.0078115*
dash.authorsorderedfalse
dash.contributor.affiliatedHochberg, Fred H
dash.contributor.affiliatedBreakefield, Xandra


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record