Show simple item record

dc.contributor.authorLemay, Danielle Gen_US
dc.contributor.authorHovey, Russell Cen_US
dc.contributor.authorHartono, Stella Ren_US
dc.contributor.authorHinde, Katieen_US
dc.contributor.authorSmilowitz, Jennifer Ten_US
dc.contributor.authorVentimiglia, Franken_US
dc.contributor.authorSchmidt, Kimberli Aen_US
dc.contributor.authorLee, Joyce WSen_US
dc.contributor.authorIslas-Trejo, Almaen_US
dc.contributor.authorSilva, Pedro Ivoen_US
dc.contributor.authorKorf, Ianen_US
dc.contributor.authorMedrano, Juan Fen_US
dc.contributor.authorBarry, Peter Aen_US
dc.contributor.authorGerman, J Bruceen_US
dc.date.accessioned2014-03-11T10:16:28Z
dc.date.issued2013en_US
dc.identifier.citationLemay, D. G., R. C. Hovey, S. R. Hartono, K. Hinde, J. T. Smilowitz, F. Ventimiglia, K. A. Schmidt, et al. 2013. “Sequencing the transcriptome of milk production: milk trumps mammary tissue.” BMC Genomics 14 (1): 872. doi:10.1186/1471-2164-14-872. http://dx.doi.org/10.1186/1471-2164-14-872.en
dc.identifier.issn1471-2164en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879290
dc.description.abstractBackground: Studies of normal human mammary gland development and function have mostly relied on cell culture, limited surgical specimens, and rodent models. Although RNA extracted from human milk has been used to assay the mammary transcriptome non-invasively, this assay has not been adequately validated in primates. Thus, the objectives of the current study were to assess the suitability of lactating rhesus macaques as a model for lactating humans and to determine whether RNA extracted from milk fractions is representative of RNA extracted from mammary tissue for the purpose of studying the transcriptome of milk-producing cells. Results: We confirmed that macaque milk contains cytoplasmic crescents and that ample high-quality RNA can be obtained for sequencing. Using RNA sequencing, RNA extracted from macaque milk fat and milk cell fractions more accurately represented RNA from mammary epithelial cells (cells that produce milk) than did RNA from whole mammary tissue. Mammary epithelium-specific transcripts were more abundant in macaque milk fat, whereas adipose or stroma-specific transcripts were more abundant in mammary tissue. Functional analyses confirmed the validity of milk as a source of RNA from milk-producing mammary epithelial cells. Conclusions: RNA extracted from the milk fat during lactation accurately portrayed the RNA profile of milk-producing mammary epithelial cells in a non-human primate. However, this sample type clearly requires protocols that minimize RNA degradation. Overall, we validated the use of RNA extracted from human and macaque milk and provided evidence to support the use of lactating macaques as a model for human lactation.en
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.relation.isversionofdoi:10.1186/1471-2164-14-872en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871720/pdf/en
dash.licenseLAAen_US
dc.subjectMammary glanden
dc.subjectMilken
dc.subjectRhesus macaqueen
dc.subjectHumanen
dc.subjectTranscriptomeen
dc.subjectRNA-Seqen
dc.subjectMilk RNAen
dc.subjectBioinformaticsen
dc.subjectMilk processingen
dc.subjectTranscriptomicsen
dc.titleSequencing the transcriptome of milk production: milk trumps mammary tissueen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalBMC Genomicsen
dc.date.available2014-03-11T10:16:28Z
dc.identifier.doi10.1186/1471-2164-14-872*
dash.authorsorderedfalse


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record