Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio
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CitationTantama, Mathew, Juan Ramón Martínez-François, Rebecca Mongeon, and Gary Yellen. 2013. “Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio.” Nature communications 4 (1): 10.1038/ncomms3550. doi:10.1038/ncomms3550. http://dx.doi.org/10.1038/ncomms3550.
AbstractThe ATP:ADP ratio is a critical parameter of cellular energy status that regulates many metabolic activities. Here we report an optimized genetically-encoded fluorescent biosensor, PercevalHR, that senses the ATP:ADP ratio. PercevalHR is tuned to the range of intracellular ATP:ADP expected in mammalian cells, and it can be used with one- or two-photon microscopy in live samples. We use PercevalHR to visualize activity-dependent changes in ATP:ADP when neurons are exposed to multiple stimuli, demonstrating that it is a sensitive reporter of physiological changes in energy consumption and production. We also use PercevalHR to visualize intracellular ATP:ADP while simultaneously recording currents from ATP-sensitive potassium (KATP) channels in single cells, showing that PercevalHR enables the study of coordinated variation in ATP:ADP and KATP channel open probability in intact cells. With its ability to monitor changes in cellular energetics within seconds, PercevalHR should be a versatile tool for metabolic research.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879321
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