Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate
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CitationMatsumoto, Hidetaka, Joan W. Miller, and Demetrios G. Vavvas. 2013. “Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate.” Journal of Visualized Experiments : JoVE (79): 50660. doi:10.3791/50660. http://dx.doi.org/10.3791/50660.
AbstractSubretinal injection of sodium hyaluronate is a widely accepted method of inducing retinal detachment (RD)1-15. However, the height and duration of RD or the occurrence of subretinal hemorrhage can affect photoreceptor cell death in the detached retina16-21. Hence, it is advantageous to create reproducible RDs without subretinal hemorrhage for evaluating photoreceptor cell death. We modified a previously reported method to create bullous and persistent RDs in a reproducible location with rare occurrence of subretinal hemorrhage. The critical step of this modified method is the creation of a self-sealing scleral incision, which can prevent leakage of sodium hyaluronate after injection into the subretinal space. To make the self-sealing scleral incision, a scleral tunnel is created, followed by scleral penetration into the choroid with a 30 G needle. Although choroidal hemorrhage may occur during this step, astriction with a surgical spear reduces the rate of choroidal hemorrhage. This method allows a more reproducible and reliable model of photoreceptor death in diseases that involve RD such as rhegmatogenous RD, retinopathy of prematurity, diabetic retinopathy, central serous chorioretinopathy, and age-related macular degeneration (AMD).
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879370
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