Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate

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Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate

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Title: Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate
Author: Matsumoto, Hidetaka; Miller, Joan W.; Vavvas, Demetrios G.

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Citation: Matsumoto, Hidetaka, Joan W. Miller, and Demetrios G. Vavvas. 2013. “Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate.” Journal of Visualized Experiments : JoVE (79): 50660. doi:10.3791/50660. http://dx.doi.org/10.3791/50660.
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Abstract: Subretinal injection of sodium hyaluronate is a widely accepted method of inducing retinal detachment (RD)1-15. However, the height and duration of RD or the occurrence of subretinal hemorrhage can affect photoreceptor cell death in the detached retina16-21. Hence, it is advantageous to create reproducible RDs without subretinal hemorrhage for evaluating photoreceptor cell death. We modified a previously reported method to create bullous and persistent RDs in a reproducible location with rare occurrence of subretinal hemorrhage. The critical step of this modified method is the creation of a self-sealing scleral incision, which can prevent leakage of sodium hyaluronate after injection into the subretinal space. To make the self-sealing scleral incision, a scleral tunnel is created, followed by scleral penetration into the choroid with a 30 G needle. Although choroidal hemorrhage may occur during this step, astriction with a surgical spear reduces the rate of choroidal hemorrhage. This method allows a more reproducible and reliable model of photoreceptor death in diseases that involve RD such as rhegmatogenous RD, retinopathy of prematurity, diabetic retinopathy, central serous chorioretinopathy, and age-related macular degeneration (AMD).
Published Version: doi:10.3791/50660
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864357/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879370
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