The transcriptional landscape of αβ T cell differentiation
von Boehmer, Harald
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CitationMingueneau, M., T. Kreslavsky, D. Gray, T. Heng, R. Cruse, J. Ericson, S. Bendall, et al. 2013. “The transcriptional landscape of αβ T cell differentiation.” Nature immunology 14 (6): 10.1038/ni.2590. doi:10.1038/ni.2590. http://dx.doi.org/10.1038/ni.2590.
AbstractαβT cell differentiation from thymic precursors is a complex process, explored here with the breadth of ImmGen expression datasets, analyzing how differentiation of thymic precursors gives rise to transcriptomes. After surprisingly gradual changes though early T commitment, transit through the CD4+CD8+ stage involves a shutdown or rare breadth, and correlating tightly with MYC. MHC-driven selection promotes a large-scale transcriptional reactivation. We identify distinct signatures that mark cells destined for positive selection versus apoptotic deletion. Differential expression of surprisingly few genes accompany CD4 or CD8 commitment, a similarity that carries through to peripheral T cells and their activation, revealed by mass cytometry phosphoproteomics. The novel transcripts identified as candidate mediators of key transitions help define the “known unknown” of thymocyte differentiation.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879434
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