Zfp36l2 is required for self-renewal of early erythroid BFU-E progenitors

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Zfp36l2 is required for self-renewal of early erythroid BFU-E progenitors

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Title: Zfp36l2 is required for self-renewal of early erythroid BFU-E progenitors
Author: Zhang, Lingbo; Prak, Lina; Rayon-Estrada, Violeta; Thiru, Prathapan; Flygare, Johan; Lim, Bing; Lodish, Harvey F.

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Citation: Zhang, Lingbo, Lina Prak, Violeta Rayon-Estrada, Prathapan Thiru, Johan Flygare, Bing Lim, and Harvey F. Lodish. 2013. “Zfp36l2 is required for self-renewal of early erythroid BFU-E progenitors.” Nature 499 (7456): 92-96. doi:10.1038/nature12215. http://dx.doi.org/10.1038/nature12215.
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Abstract: Stem cells and progenitors in many lineages undergo self- renewing divisions, but the extracellular and intracellular proteins that regulate this process are largely unknown. Glucocorticoids stimulate red cell formation by promoting self-renewal of early erythroid burst forming unit-erythrocyte (BFU-E) progenitors1-4. Here we show that the RNA binding protein Zfp36l2 is a transcriptional target of the glucocorticoid receptor (GR) in BFU-Es and is required for BFU-E self-renewal. Zfp36l2 is normally downregulated during erythroid differentiation from the BFU-E stage but its expression is maintained by all tested GR agonists that stimulate BFU-E self-renewal, and the GR binds to several potential enhancer regions of Zfp36l2. Knockdown of Zfp36l2 in cultured BFU-E cells did not affect the rate of cell division but disrupted glucocorticoid-induced BFU-E self-renewal, and knockdown of Zfp36l2 in transplanted erythroid progenitors prevented expansion of erythroid lineage progenitors normally seen following induction of anemia by phenylhydrazine treatment. Zfp36l2 preferentially binds to mRNAs that are induced or maintained at high expression levels during terminal erythroid differentiation and negatively regulates their expression levels. Thus Zfp36l2 functions as part of molecular switch promoting BFU-E self-renewal and thus a subsequent increase in the total numbers of CFU-E progenitors and erythroid cells that are generated.
Published Version: doi:10.1038/nature12215
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702661/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879584
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