A Screen for Morphological Complexity Identifies Regulators of Switch-like Transitions between Discrete Cell Shapes

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A Screen for Morphological Complexity Identifies Regulators of Switch-like Transitions between Discrete Cell Shapes

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Title: A Screen for Morphological Complexity Identifies Regulators of Switch-like Transitions between Discrete Cell Shapes
Author: Yin, Zheng; Sadok, Amine; Sailem, Heba; McCarthy, Afshan; Xia, Xiaofeng; Li, Fuhai; Garcia, Mar Arias; Evans, Louise; Barr, Alexis; Perrimon, Norbert; Marshall, Christopher J; Wong, Stephen T.C.; Bakal, Chris

Note: Order does not necessarily reflect citation order of authors.

Citation: Yin, Z., A. Sadok, H. Sailem, A. McCarthy, X. Xia, F. Li, M. A. Garcia, et al. 2013. “A Screen for Morphological Complexity Identifies Regulators of Switch-like Transitions between Discrete Cell Shapes.” Nature cell biology 15 (7): 860-871. doi:10.1038/ncb2764. http://dx.doi.org/10.1038/ncb2764.
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Abstract: The way in which cells adopt different morphologies is not fully understood. Cell shape could be a continuous variable or restricted to a set of discrete forms. We developed quantitative methods to describe cell shape and show that Drosophila hemocytes in culture are a heterogeneous mixture of five discrete morphologies. In an RNAi screen of genes affecting the morphological complexity of heterogeneous populations, we found that most genes regulate the transition between discrete shapes rather than generating new morphologies. In particular, we identified a subset of genes, including the tumour suppressor PTEN, that decrease the heterogeneity of the population leading to populations enriched in rounded or elongated forms. We show that these genes have a highly conserved function as regulators of cell shape in both mouse and human metastatic melanoma cells.
Published Version: doi:10.1038/ncb2764
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712499/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879609
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