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dc.contributor.authorBalasubramanian, Deepaken_US
dc.contributor.authorKumari, Hansien_US
dc.contributor.authorJaric, Melitaen_US
dc.contributor.authorFernandez, Mitchen_US
dc.contributor.authorTurner, Keith H.en_US
dc.contributor.authorDove, Simon L.en_US
dc.contributor.authorNarasimhan, Girien_US
dc.contributor.authorLory, Stephenen_US
dc.contributor.authorMathee, Kalaien_US
dc.date.accessioned2014-03-11T13:27:22Z
dc.date.issued2013en_US
dc.identifier.citationBalasubramanian, Deepak, Hansi Kumari, Melita Jaric, Mitch Fernandez, Keith H. Turner, Simon L. Dove, Giri Narasimhan, Stephen Lory, and Kalai Mathee. 2013. “Deep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat shock and oxidative stress response.” Nucleic Acids Research 42 (2): 979-998. doi:10.1093/nar/gkt942. http://dx.doi.org/10.1093/nar/gkt942.en
dc.identifier.issn0305-1048en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879653
dc.description.abstractPathogenicity of Pseudomonas aeruginosa, a major cause of many acute and chronic human infections, is determined by tightly regulated expression of multiple virulence factors. Quorum sensing (QS) controls expression of many of these pathogenic determinants. Previous microarray studies have shown that the AmpC β-lactamase regulator AmpR, a member of the LysR family of transcription factors, also controls non-β-lactam resistance and multiple virulence mechanisms. Using RNA-Seq and complementary assays, this study further expands the AmpR regulon to include diverse processes such as oxidative stress, heat shock and iron uptake. Importantly, AmpR affects many of these phenotypes, in part, by regulating expression of non-coding RNAs such as rgP32, asRgsA, asPrrF1 and rgRsmZ. AmpR positively regulates expression of the major QS regulators LasR, RhlR and MvfR, and genes of the Pseudomonas quinolone system. Chromatin immunoprecipitation (ChIP)-Seq and ChIP–quantitative real-time polymerase chain reaction studies show that AmpR binds to the ampC promoter both in the absence and presence of β-lactams. In addition, AmpR directly binds the lasR promoter, encoding the QS master regulator. Comparison of the AmpR-binding sequences from the transcriptome and ChIP-Seq analyses identified an AT-rich consensus-binding motif. This study further attests to the role of AmpR in regulating virulence and physiological processes in P. aeruginosa.en
dc.language.isoen_USen
dc.publisherOxford University Pressen
dc.relation.isversionofdoi:10.1093/nar/gkt942en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3902932/pdf/en
dash.licenseLAAen_US
dc.titleDeep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat shock and oxidative stress responseen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNucleic Acids Researchen
dash.depositing.authorBalasubramanian, Deepaken_US
dc.date.available2014-03-11T13:27:22Z
dc.identifier.doi10.1093/nar/gkt942*
dash.contributor.affiliatedBalasubramanian, Deepak
dash.contributor.affiliatedLory, Stephen
dash.contributor.affiliatedDove, Simon


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