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dc.contributor.authorHara, Emilio Satoshien_US
dc.contributor.authorOno, Mitsuakien_US
dc.contributor.authorEguchi, Takanorien_US
dc.contributor.authorKubota, Satoshien_US
dc.contributor.authorPham, Hai Thanhen_US
dc.contributor.authorSonoyama, Wataruen_US
dc.contributor.authorTajima, Shojien_US
dc.contributor.authorTakigawa, Masaharuen_US
dc.contributor.authorCalderwood, Stuart K.en_US
dc.contributor.authorKuboki, Takuoen_US
dc.date.accessioned2014-03-11T13:27:47Z
dc.date.issued2013en_US
dc.identifier.citationHara, Emilio Satoshi, Mitsuaki Ono, Takanori Eguchi, Satoshi Kubota, Hai Thanh Pham, Wataru Sonoyama, Shoji Tajima, Masaharu Takigawa, Stuart K. Calderwood, and Takuo Kuboki. 2013. “miRNA-720 Controls Stem Cell Phenotype, Proliferation and Differentiation of Human Dental Pulp Cells.” PLoS ONE 8 (12): e83545. doi:10.1371/journal.pone.0083545. http://dx.doi.org/10.1371/journal.pone.0083545.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879707
dc.description.abstractDental pulp cells (DPCs) are known to be enriched in stem/progenitor cells but not well characterized yet. Small non-coding microRNAs (miRNAs) have been identified to control protein translation, mRNA stability and transcription, and have been reported to play important roles in stem cell biology, related to cell reprogramming, maintenance of stemness and regulation of cell differentiation. In order to characterize dental pulp stem/progenitor cells and its mechanism of differentiation, we herein sorted stem-cell-enriched side population (SP) cells from human DPCs and periodontal ligament cells (PDLCs), and performed a locked nucleic acid (LNA)-based miRNA array. As a result, miR-720 was highly expressed in the differentiated main population (MP) cells compared to that in SP cells. In silico analysis and a reporter assay showed that miR-720 targets the stem cell marker NANOG, indicating that miR-720 could promote differentiation of dental pulp stem/progenitor cells by repressing NANOG. Indeed, gain-and loss-of-function analyses showed that miR-720 controls NANOG transcript and protein levels. Moreover, transfection of miR-720 significantly decreased the number of cells positive for the early stem cell marker SSEA-4. Concomitantly, mRNA levels of DNA methyltransferases (DNMTs), which are known to play crucial factors during stem cell differentiation, were also increased by miR-720 through unknown mechanism. Finally, miR-720 decreased DPC proliferation as determined by immunocytochemical analysis against ki-67, and promoted odontogenic differentiation as demonstrated by alizarin red staining, as well as alkaline phosphatase and osteopontin mRNA levels. Our findings identify miR-720 as a novel miRNA regulating the differentiation of DPCs.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0083545en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875457/pdf/en
dash.licenseLAAen_US
dc.subjectBiologyen
dc.subjectBiochemistryen
dc.subjectNucleic Acidsen
dc.subjectRNAen
dc.subjectRNA interferenceen
dc.subjectDevelopmental Biologyen
dc.subjectCell Differentiationen
dc.subjectMolecular Cell Biologyen
dc.subjectCellular Typesen
dc.subjectStem Cellsen
dc.subjectAdult Stem Cellsen
dc.subjectMedicineen
dc.subjectOral Medicineen
dc.subjectDentistryen
dc.titlemiRNA-720 Controls Stem Cell Phenotype, Proliferation and Differentiation of Human Dental Pulp Cellsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorEguchi, Takanorien_US
dc.date.available2014-03-11T13:27:47Z
dc.identifier.doi10.1371/journal.pone.0083545*
dash.contributor.affiliatedEguchi, Takanori
dash.contributor.affiliatedCalderwood, Stuart


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