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dc.contributor.authorWu, Qiongen_US
dc.contributor.authorWang, Ruien_US
dc.contributor.authorYang, Qinglingen_US
dc.contributor.authorHou, Xinen_US
dc.contributor.authorChen, Sulianen_US
dc.contributor.authorHou, Yueyueen_US
dc.contributor.authorChen, Changjieen_US
dc.contributor.authorYang, Yanen_US
dc.contributor.authorMiele, Lucioen_US
dc.contributor.authorSarkar, Fazlul Hen_US
dc.contributor.authorChen, Yuqingen_US
dc.contributor.authorWang, Zhiweien_US
dc.date.accessioned2014-03-11T13:27:48Z
dc.date.issued2013en_US
dc.identifier.citationWu, Q., R. Wang, Q. Yang, X. Hou, S. Chen, Y. Hou, C. Chen, et al. 2013. “Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway.” Oncotarget 4 (11): 1999-2009.en
dc.identifier.issn1949-2553en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879708
dc.description.abstractHepatocellular carcinoma (HCC) is one of the common malignances in the world and has high mortality in part due to development of acquired drug resistance. Therefore, it is urgent to investigate the molecular mechanism of drug resistance in HCC. To explore the underlying mechanism of drug resistance in HCC, we developed gemcitabine-resistant (GR) HCC cells. We used multiple methods to achieve our goal including RT-PCR, Western blotting analysis, transfection, Wound-healing assay, migration and invasion assay. We observed that gemcitabine-resistant cells acquired epithelial-mesenchymal transition (EMT) phenotype. Moreover, we found that PDGF-D is highly expressed in GR cells. Furthermore, down-regulation of PDGF-D in GR cells led to partial reversal of the EMT phenotype. Our findings demonstrated that targeting PDGF-D could be a novel strategy to overcome gemcitabine resistance in HCC.en
dc.language.isoen_USen
dc.publisherImpact Journals LLCen
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875765/pdf/en
dash.licenseLAAen_US
dc.subjectHepatocellular carcinomaen
dc.subjectchemoresistanceen
dc.subjectPDGF-Den
dc.subjectEMTen
dc.subjectgemcitabineen
dc.titleChemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathwayen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalOncotargeten
dc.date.available2014-03-11T13:27:48Z
dc.identifier.doi10.18632/oncotarget.1471
dash.authorsorderedfalse


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