Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos
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Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos
| Title: | Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos |
| Author: |
Gisselbrecht, Stephen S.; Barrera, Luis A.; Porsch, Martin; Aboukhalil, Anton; Estep, Preston W.; Vedenko, Anastasia; Palagi, Alexandre; Kim, Yongsok; Zhu, Xianmin; Busser, Brian W.; Gamble, Caitlin E.; Iagovitina, Antonina; Singhania, Aditi; Michelson, Alan M.; Bulyk, Martha L.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Gisselbrecht, S. S., L. A. Barrera, M. Porsch, A. Aboukhalil, P. W. Estep, A. Vedenko, A. Palagi, et al. 2013. “Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos.” Nature methods 10 (8): 774-780. doi:10.1038/nmeth.2558. http://dx.doi.org/10.1038/nmeth.2558. |
| Full Text & Related Files: |
3733245.pdf (3.890Mb; PDF) 
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| Abstract: | Transcriptional enhancers are a primary mechanism by which tissue-specific gene expression is achieved. Despite the importance of these regulatory elements in development, responses to environmental stresses, and disease, testing enhancer activity in animals remains tedious, with a minority of enhancers having been characterized. Here, we have developed ‘enhancer-FACS-Seq’ (eFS) technology for highly parallel identification of active, tissue-specific enhancers in Drosophila embryos. Analysis of enhancers identified by eFS to be active in mesodermal tissues revealed enriched DNA binding site motifs of known and putative, novel mesodermal transcription factors (TFs). Naïve Bayes classifiers using TF binding site motifs accurately predicted mesodermal enhancer activity. Application of eFS to other cell types and organisms should accelerate the cataloging of enhancers and understanding how transcriptional regulation is encoded within them. |
| Published Version: | doi:10.1038/nmeth.2558 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733245/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879734 |
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