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dc.contributor.authorCarter, Ava C.en_US
dc.contributor.authorDavis-Dusenbery, Brandi N.en_US
dc.contributor.authorKoszka, Kathrynen_US
dc.contributor.authorIchida, Justin K.en_US
dc.contributor.authorEggan, Kevinen_US
dc.date.accessioned2014-03-11T13:51:50Z
dc.date.issued2014en_US
dc.identifier.citationCarter, Ava C., Brandi N. Davis-Dusenbery, Kathryn Koszka, Justin K. Ichida, and Kevin Eggan. 2014. “Nanog-Independent Reprogramming to iPSCs with Canonical Factors.” Stem Cell Reports 2 (2): 119-126. doi:10.1016/j.stemcr.2013.12.010. http://dx.doi.org/10.1016/j.stemcr.2013.12.010.en
dc.identifier.issn2213-6711en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879736
dc.description.abstractSummary It has been suggested that the transcription factor Nanog is essential for the establishment of pluripotency during the derivation of embryonic stem cells and induced pluripotent stem cells (iPSCs). However, successful reprogramming to pluripotency with a growing list of divergent transcription factors, at ever-increasing efficiencies, suggests that there may be many distinct routes to a pluripotent state. Here, we have investigated whether Nanog is necessary for reprogramming murine fibroblasts under highly efficient conditions using the canonical-reprogramming factors Oct4, Sox2, Klf4, and cMyc. In agreement with prior results, the efficiency of reprogramming Nanog−/− fibroblasts was significantly lower than that of control fibroblasts. However, in contrast to previous findings, we were able to reproducibly generate iPSCs from Nanog−/− fibroblasts that effectively contributed to the germline of chimeric mice. Thus, whereas Nanog may be an important mediator of reprogramming, it is not required for establishing pluripotency in the mouse, even under standard conditions.en
dc.language.isoen_USen
dc.publisherElsevieren
dc.relation.isversionofdoi:10.1016/j.stemcr.2013.12.010en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923195/pdf/en
dash.licenseLAAen_US
dc.titleNanog-Independent Reprogramming to iPSCs with Canonical Factorsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalStem Cell Reportsen
dash.depositing.authorKoszka, Kathrynen_US
dc.date.available2014-03-11T13:51:50Z
dc.identifier.doi10.1016/j.stemcr.2013.12.010*
dash.contributor.affiliatedKoszka, Kathryn
dash.contributor.affiliatedEggan, Kevin


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