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dc.contributor.authorNalabothula, Narasimharaoen_US
dc.contributor.authorMcVicker, Grahamen_US
dc.contributor.authorMaiorano, Johnen_US
dc.contributor.authorMartin, Rebeccaen_US
dc.contributor.authorPritchard, Jonathan Ken_US
dc.contributor.authorFondufe-Mittendorf, Yvonne Nen_US
dc.date.accessioned2014-03-11T13:52:41Z
dc.date.issued2014en_US
dc.identifier.citationNalabothula, Narasimharao, Graham McVicker, John Maiorano, Rebecca Martin, Jonathan K Pritchard, and Yvonne N Fondufe-Mittendorf. 2014. “The chromatin architectural proteins HMGD1 and H1 bind reciprocally and have opposite effects on chromatin structure and gene regulation.” BMC Genomics 15 (1): 92. doi:10.1186/1471-2164-15-92. http://dx.doi.org/10.1186/1471-2164-15-92.en
dc.identifier.issn1471-2164en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11879790
dc.description.abstractBackground: Chromatin architectural proteins interact with nucleosomes to modulate chromatin accessibility and higher-order chromatin structure. While these proteins are almost certainly important for gene regulation they have been studied far less than the core histone proteins. Results: Here we describe the genomic distributions and functional roles of two chromatin architectural proteins: histone H1 and the high mobility group protein HMGD1 in Drosophila S2 cells. Using ChIP-seq, biochemical and gene specific approaches, we find that HMGD1 binds to highly accessible regulatory chromatin and active promoters. In contrast, H1 is primarily associated with heterochromatic regions marked with repressive histone marks. We find that the ratio of HMGD1 to H1 binding is a better predictor of gene activity than either protein by itself, which suggests that reciprocal binding between these proteins is important for gene regulation. Using knockdown experiments, we show that HMGD1 and H1 affect the occupancy of the other protein, change nucleosome repeat length and modulate gene expression. Conclusion: Collectively, our data suggest that dynamic and mutually exclusive binding of H1 and HMGD1 to nucleosomes and their linker sequences may control the fluid chromatin structure that is required for transcriptional regulation. This study provides a framework to further study the interplay between chromatin architectural proteins and epigenetics in gene regulation.en
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.relation.isversionofdoi:10.1186/1471-2164-15-92en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928079/pdf/en
dash.licenseLAAen_US
dc.subjectChromatin structureen
dc.subjectTranscriptional regulationen
dc.subjectHistone H1en
dc.subjectHigh mobility group proteinen
dc.subjectNucleosome repeat lengthen
dc.titleThe chromatin architectural proteins HMGD1 and H1 bind reciprocally and have opposite effects on chromatin structure and gene regulationen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalBMC Genomicsen
dash.depositing.authorMcVicker, Grahamen_US
dc.date.available2014-03-11T13:52:41Z
dc.identifier.doi10.1186/1471-2164-15-92*
dash.contributor.affiliatedMcVicker, Graham


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