Zfp322a Regulates Mouse ES Cell Pluripotency and Enhances Reprogramming Efficiency

DSpace/Manakin Repository

Zfp322a Regulates Mouse ES Cell Pluripotency and Enhances Reprogramming Efficiency

Citable link to this page


Title: Zfp322a Regulates Mouse ES Cell Pluripotency and Enhances Reprogramming Efficiency
Author: Ma, Hui; Ng, Hui Min; Teh, Xiuwen; Li, Hu; Lee, Yun Hwa; Chong, Yew Mei; Loh, Yuin Han; Collins, James J.; Feng, Bo; Yang, Henry; Wu, Qiang

Note: Order does not necessarily reflect citation order of authors.

Citation: Ma, H., H. M. Ng, X. Teh, H. Li, Y. H. Lee, Y. M. Chong, Y. H. Loh, et al. 2014. “Zfp322a Regulates Mouse ES Cell Pluripotency and Enhances Reprogramming Efficiency.” PLoS Genetics 10 (2): e1004038. doi:10.1371/journal.pgen.1004038. http://dx.doi.org/10.1371/journal.pgen.1004038.
Full Text & Related Files:
Abstract: Embryonic stem (ES) cells derived from the inner cell mass (ICM) of blastocysts are characterised by their ability to self-renew and their potential to differentiate into many different cell types. Recent studies have shown that zinc finger proteins are crucial for maintaining pluripotent ES cells. Mouse zinc finger protein 322a (Zfp322a) is expressed in the ICM of early mouse embryos. However, little is known regarding the role of Zfp322a in the pluripotency maintenance of mouse ES cells. Here, we report that Zfp322a is required for mES cell identity since depletion of Zfp322a directs mES cells towards differentiation. Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays revealed that Zfp322a binds to Pou5f1 and Nanog promoters and regulates their transcription. These data along with the results obtained from our ChIP-seq experiment showed that Zfp322a is an essential component of mES cell transcription regulatory network. Targets which are directly regulated by Zfp322a were identified by correlating the gene expression profile of Zfp322a RNAi-treated mES cells with the ChIP-seq results. These experiments revealed that Zfp322a inhibits mES cell differentiation by suppressing MAPK pathway. Additionally, Zfp322a is found to be a novel reprogramming factor that can replace Sox2 in the classical Yamanaka's factors (OSKM). It can be even used in combination with Yamanaka's factors and that addition leads to a higher reprogramming efficiency and to acceleration of the onset of the reprogramming process. Together, our results demonstrate that Zfp322a is a novel essential component of the transcription factor network which maintains the identity of mouse ES cells.
Published Version: doi:10.1371/journal.pgen.1004038
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923668/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879859
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search