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dc.contributor.authorTabansky, Inna
dc.contributor.authorLenarcic, Alan
dc.contributor.authorDraft, Ryan Wesley
dc.contributor.authorLoulier, Karine
dc.contributor.authorKeskin, Derin Benerci
dc.contributor.authorRosains, Jacqueline
dc.contributor.authorRivera-Feliciano, Jose
dc.contributor.authorLichtman, Jeff
dc.contributor.authorLivet, Jean
dc.contributor.authorStern, Joel N H
dc.contributor.authorSanes, Joshua R.
dc.contributor.authorEggan, Kevin Carl
dc.date.accessioned2014-03-12T12:09:22Z
dc.date.issued2013
dc.identifierQuick submit: 2014-03-11T23:34:58-04:00
dc.identifier.citationTabansky, Inna, Alan Lenarcic, Ryan W. Draft, Karine Loulier, Derin B. Keskin, Jacqueline Rosains, José Rivera-Feliciano, et al. 2013. “Developmental Bias in Cleavage-Stage Mouse Blastomeres.” Current Biology 23 (1) (January): 21–31. doi:10.1016/j.cub.2012.10.054. http://dx.doi.org/10.1016/j.cub.2012.10.054.en_US
dc.identifier.issn0960-9822en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11880286
dc.description.abstractBACKGROUND: The cleavage-stage mouse embryo is composed of superficially equivalent blastomeres that will generate both the embryonic inner cell mass (ICM) and the supportive trophectoderm (TE). However, it remains unsettled whether the contribution of each blastomere to these two lineages can be accounted for by chance. Addressing the question of blastomere cell fate may be of practical importance, because preimplantation genetic diagnosis requires removal of blastomeres from the early human embryo. To determine whether blastomere allocation to the two earliest lineages is random, we developed and utilized a recombination-mediated, noninvasive combinatorial fluorescent labeling method for embryonic lineage tracing. RESULTS: When we induced recombination at cleavage stages, we observed a statistically significant bias in the contribution of the resulting labeled clones to the trophectoderm or the inner cell mass in a subset of embryos. Surprisingly, we did not find a correlation between localization of clones in the embryonic and abembryonic hemispheres of the late blastocyst and their allocation to the TE and ICM, suggesting that TE-ICM bias arises separately from embryonic-abembryonic bias. Rainbow lineage tracing also allowed us to demonstrate that the bias observed in the blastocyst persists into postimplantation stages and therefore has relevance for subsequent development. CONCLUSIONS: The Rainbow transgenic mice that we describe here have allowed us to detect lineage-dependent bias in early development. They should also enable assessment of the developmental equivalence of mammalian progenitor cells in a variety of tissues.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherElsevier BVen_US
dc.relation.isversionofdoi:10.1016/j.cub.2012.10.054en_US
dash.licenseOAP
dc.titleDevelopmental Bias in Cleavage-Stage Mouse Blastomeresen_US
dc.typeJournal Articleen_US
dc.date.updated2014-03-12T03:36:47Z
dc.description.versionAccepted Manuscripten_US
dc.rights.holderJeff Lichtman
dc.relation.journalCurrent Biologyen_US
dash.depositing.authorEggan, Kevin Carl
dc.date.available2014-03-12T12:09:22Z
dc.identifier.doi10.1016/j.cub.2012.10.054*
dash.authorsorderedfalse
dash.contributor.affiliatedRivera-Feliciano, Jose
dash.contributor.affiliatedDraft, Ryan
dash.contributor.affiliatedRosains, Jacqueline
dash.contributor.affiliatedStern, Joel N H
dash.contributor.affiliatedSanes, Joshua
dash.contributor.affiliatedKeskin, Derin Benerci
dash.contributor.affiliatedLichtman, Jeff
dash.contributor.affiliatedEggan, Kevin


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