Measuring Markers of Liver Function Using a Micropatterned Paper Device Designed for Blood from a Fingerstick
Vella, Sarah J.
Martinez, Andres W.
Phillips, Scott T.
Mirica, Katherine A.
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CitationVella, Sarah J., Patrick Beattie, Rebecca Cademartiri, Anna Laromaine, Andres W. Martinez, Scott T. Phillips, Katherine A. Mirica, and George M. Whitesides. 2012. Measuring Markers of Liver Function Using a Micropatterned Paper Device Designed for Blood from a Fingerstick. Analytical Chemistry 84, no. 6: 2883–2891.
AbstractThis paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and “spiked” standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:11956907
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