Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscle

DSpace/Manakin Repository

Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscle

Citable link to this page

 

 
Title: Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscle
Author: Castiglioni, Alessandra; Hettmer, Simone; Lynes, Matthew D.; Rao, Tata Nageswara; Tchessalova, Daria; Sinha, Indranil; Lee, Bernard T.; Tseng, Yu-Hua; Wagers, Amy J.

Note: Order does not necessarily reflect citation order of authors.

Citation: Castiglioni, Alessandra, Simone Hettmer, Matthew D. Lynes, Tata Nageswara Rao, Daria Tchessalova, Indranil Sinha, Bernard T. Lee, Yu-Hua Tseng, and Amy J. Wagers. 2014. “Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscle.” Stem Cell Reports 2 (1): 92-106. doi:10.1016/j.stemcr.2013.12.006. http://dx.doi.org/10.1016/j.stemcr.2013.12.006.
Full Text & Related Files:
Abstract: Summary Fluorescence-activated cell sorting (FACS) strategies to purify distinct cell types from the pool of fetal human myofiber-associated (hMFA) cells were developed. We demonstrate that cells expressing the satellite cell marker PAX7 are highly enriched within the subset of CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi hMFA cells. These CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi cells lack adipogenic capacity but exhibit robust, bipotent myogenic and osteogenic activity in vitro and engraft myofibers when transplanted into mouse muscle. In contrast, CD45−CD11b−GlyA−CD31−CD34+ fetal hMFA cells represent stromal constituents of muscle that do not express PAX7, lack myogenic function, and exhibit adipogenic and osteogenic capacity in vitro. Adult muscle likewise contains PAX7+ CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi hMFA cells with in vitro myogenic and osteogenic activity, although these cells are present at lower frequency in comparison to their fetal counterparts. The ability to directly isolate functionally distinct progenitor cells from human muscle will enable novel insights into muscle lineage specification and homeostasis.
Published Version: doi:10.1016/j.stemcr.2013.12.006
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966115/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12064357
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters