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dc.contributor.authorCastiglioni, Alessandraen_US
dc.contributor.authorHettmer, Simoneen_US
dc.contributor.authorLynes, Matthew D.en_US
dc.contributor.authorRao, Tata Nageswaraen_US
dc.contributor.authorTchessalova, Dariaen_US
dc.contributor.authorSinha, Indranilen_US
dc.contributor.authorLee, Bernard T.en_US
dc.contributor.authorTseng, Yu-Huaen_US
dc.contributor.authorWagers, Amy J.en_US
dc.date.accessioned2014-04-11T14:10:41Z
dc.date.issued2014en_US
dc.identifier.citationCastiglioni, Alessandra, Simone Hettmer, Matthew D. Lynes, Tata Nageswara Rao, Daria Tchessalova, Indranil Sinha, Bernard T. Lee, Yu-Hua Tseng, and Amy J. Wagers. 2014. “Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscle.” Stem Cell Reports 2 (1): 92-106. doi:10.1016/j.stemcr.2013.12.006. http://dx.doi.org/10.1016/j.stemcr.2013.12.006.en
dc.identifier.issn2213-6711en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12064357
dc.description.abstractSummary Fluorescence-activated cell sorting (FACS) strategies to purify distinct cell types from the pool of fetal human myofiber-associated (hMFA) cells were developed. We demonstrate that cells expressing the satellite cell marker PAX7 are highly enriched within the subset of CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi hMFA cells. These CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi cells lack adipogenic capacity but exhibit robust, bipotent myogenic and osteogenic activity in vitro and engraft myofibers when transplanted into mouse muscle. In contrast, CD45−CD11b−GlyA−CD31−CD34+ fetal hMFA cells represent stromal constituents of muscle that do not express PAX7, lack myogenic function, and exhibit adipogenic and osteogenic capacity in vitro. Adult muscle likewise contains PAX7+ CD45−CD11b−GlyA−CD31−CD34−CD56intITGA7hi hMFA cells with in vitro myogenic and osteogenic activity, although these cells are present at lower frequency in comparison to their fetal counterparts. The ability to directly isolate functionally distinct progenitor cells from human muscle will enable novel insights into muscle lineage specification and homeostasis.en
dc.language.isoen_USen
dc.publisherElsevieren
dc.relation.isversionofdoi:10.1016/j.stemcr.2013.12.006en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966115/pdf/en
dash.licenseLAAen_US
dc.titleIsolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscleen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalStem Cell Reportsen
dash.depositing.authorCastiglioni, Alessandraen_US
dc.date.available2014-04-11T14:10:41Z
dc.identifier.doi10.1016/j.stemcr.2013.12.006*
dash.contributor.affiliatedTchessalova, Daria
dash.contributor.affiliatedSinha, Indranil
dash.contributor.affiliatedTseng, Yu-Hua
dash.contributor.affiliatedCastiglioni, Alessandra


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