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dc.contributor.authorKrause, Silvaen_US
dc.contributor.authorBrock, Amyen_US
dc.contributor.authorIngber, Donald E.en_US
dc.date.accessioned2014-04-11T14:10:43Z
dc.date.issued2013en_US
dc.identifier.citationKrause, Silva, Amy Brock, and Donald E. Ingber. 2013. “Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland.” Journal of Visualized Experiments : JoVE (80): 50692. doi:10.3791/50692. http://dx.doi.org/10.3791/50692.en
dc.identifier.issn1940-087Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12064361
dc.description.abstractHerein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of genes within the mammary epithelium has been difficult to achieve due to the lack of appropriate targeting molecules that are independent of developmental stages such as pregnancy and lactation. Herein, we describe a technique for localized delivery of reagents to the mammary gland at any stage in adulthood via intraductal injection into the nipples of mice. The injections can be performed on live mice, under anesthesia, and allow for a non-invasive and localized drug delivery to the mammary gland. Furthermore, the injections can be repeated over several months without damaging the nipple. Vital dyes such as Evans Blue are very helpful to learn the technique. Upon intraductal injection of the blue dye, the entire ductal tree becomes visible to the eye. Furthermore, fluorescently labeled reagents also allow for visualization and distribution within the mammary gland. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents, and small molecules.en
dc.language.isoen_USen
dc.publisherMyJove Corporationen
dc.relation.isversionofdoi:10.3791/50692en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938324/pdf/en
dash.licenseLAAen_US
dc.subjectDevelopmental Biologyen
dc.subjectIssue 80en
dc.subjectMammary Glandsen
dc.subjectAnimalen
dc.subjectDrug Administration Routesen
dc.subjectintraductal injectionen
dc.subjectlocal drug deliveryen
dc.subjectsiRNAen
dc.titleIntraductal Injection for Localized Drug Delivery to the Mouse Mammary Glanden
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalJournal of Visualized Experiments : JoVEen
dash.depositing.authorIngber, Donald E.en_US
dc.date.available2014-04-11T14:10:43Z
dc.identifier.doi10.3791/50692*
dash.contributor.affiliatedIngber, Donald


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