MARIS: Method for Analyzing RNA following Intracellular Sorting

DSpace/Manakin Repository

MARIS: Method for Analyzing RNA following Intracellular Sorting

Citable link to this page

 

 
Title: MARIS: Method for Analyzing RNA following Intracellular Sorting
Author: Hrvatin, Siniša; Deng, Francis; O'Donnell, Charles W.; Gifford, David K.; Melton, Douglas A.

Note: Order does not necessarily reflect citation order of authors.

Citation: Hrvatin, Siniša, Francis Deng, Charles W. O'Donnell, David K. Gifford, and Douglas A. Melton. 2014. “MARIS: Method for Analyzing RNA following Intracellular Sorting.” PLoS ONE 9 (3): e89459. doi:10.1371/journal.pone.0089459. http://dx.doi.org/10.1371/journal.pone.0089459.
Full Text & Related Files:
Abstract: Transcriptional profiling is a key technique in the study of cell biology that is limited by the availability of reagents to uniquely identify specific cell types and isolate high quality RNA from them. We report a Method for Analyzing RNA following Intracellular Sorting (MARIS) that generates high quality RNA for transcriptome profiling following cellular fixation, intracellular immunofluorescent staining and FACS. MARIS can therefore be used to isolate high quality RNA from many otherwise inaccessible cell types simply based on immunofluorescent tagging of unique intracellular proteins. As proof of principle, we isolate RNA from sorted human embryonic stem cell-derived insulin-expressing cells as well as adult human β cells. MARIS is a basic molecular biology technique that could be used across several biological disciplines.
Published Version: doi:10.1371/journal.pone.0089459
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940959/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12064364
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters