Post-Translational Regulation via Clp Protease Is Critical for Survival of Mycobacterium tuberculosis

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Post-Translational Regulation via Clp Protease Is Critical for Survival of Mycobacterium tuberculosis

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Title: Post-Translational Regulation via Clp Protease Is Critical for Survival of Mycobacterium tuberculosis
Author: Raju, Ravikiran M.; Jedrychowski, Mark P.; Wei, Jun-Rong; Pinkham, Jessica T.; Park, Annie S.; O'Brien, Kathryn; Rehren, German; Schnappinger, Dirk; Gygi, Steven P.; Rubin, Eric J.

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Citation: Raju, Ravikiran M., Mark P. Jedrychowski, Jun-Rong Wei, Jessica T. Pinkham, Annie S. Park, Kathryn O'Brien, German Rehren, Dirk Schnappinger, Steven P. Gygi, and Eric J. Rubin. 2014. “Post-Translational Regulation via Clp Protease Is Critical for Survival of Mycobacterium tuberculosis.” PLoS Pathogens 10 (3): e1003994. doi:10.1371/journal.ppat.1003994. http://dx.doi.org/10.1371/journal.ppat.1003994.
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Abstract: Unlike most bacterial species, Mycobacterium tuberculosis depends on the Clp proteolysis system for survival even in in vitro conditions. We hypothesized that Clp is required for the physiologic turnover of mycobacterial proteins whose accumulation is deleterious to bacterial growth and survival. To identify cellular substrates, we employed quantitative proteomics and transcriptomics to identify the set of proteins that accumulated upon the loss of functional Clp protease. Among the set of potential Clp substrates uncovered, we were able to unambiguously identify WhiB1, an essential transcriptional repressor capable of auto-repression, as a substrate of the mycobacterial Clp protease. Dysregulation of WhiB1 turnover had a toxic effect that was not rescued by repression of whiB1 transcription. Thus, under normal growth conditions, Clp protease is the predominant regulatory check on the levels of potentially toxic cellular proteins. Our findings add to the growing evidence of how post-translational regulation plays a critical role in the regulation of bacterial physiology.
Published Version: doi:10.1371/journal.ppat.1003994
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946367/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12064413
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