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dc.contributor.authorCiocâlteu, Adrianaen_US
dc.contributor.authorSăftoiu, Adrianen_US
dc.contributor.authorCârţână, Tatianaen_US
dc.contributor.authorGruionu, Lucian Gheorgheen_US
dc.contributor.authorPirici, Danielen_US
dc.contributor.authorGeorgescu, Corneliu Cristianen_US
dc.contributor.authorGeorgescu, Claudia-Valentinaen_US
dc.contributor.authorGheonea, Dan Ionuţen_US
dc.contributor.authorGruionu, Gabrielen_US
dc.date.accessioned2014-04-11T14:12:11Z
dc.date.issued2014en_US
dc.identifier.citationCiocâlteu, Adriana, Adrian Săftoiu, Tatiana Cârţână, Lucian Gheorghe Gruionu, Daniel Pirici, Corneliu Cristian Georgescu, Claudia-Valentina Georgescu, Dan Ionuţ Gheonea, and Gabriel Gruionu. 2014. “Evaluation of New Morphometric Parameters of Neoangiogenesis in Human Colorectal Cancer Using Confocal Laser Endomicroscopy (CLE) and Targeted Panendothelial Markers.” PLoS ONE 9 (3): e91084. doi:10.1371/journal.pone.0091084. http://dx.doi.org/10.1371/journal.pone.0091084.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12064443
dc.description.abstractThe tumor microcirculation is characterized by an abnormal vascular network with dilated, tortuous and saccular vessels. Therefore, imaging the tumor vasculature and determining its morphometric characteristics represent a critical goal for optimizing the cancer treatment that targets the blood vessels (i.e. antiangiogenesis therapy). The aim of this study was to evaluate new vascular morphometric parameters in colorectal cancer, difficult to achieve through conventional immunohistochemistry, by using the confocal laser endomicroscopy method. Fresh biopsies from tumor and normal tissue were collected during colonoscopy from five patients with T3 colorectal carcinoma without metastasis and were marked with fluorescently labeled anti-CD31 antibodies. A series of optical slices spanning 250 µm inside the tissue were immediately collected for each sample using a confocal laser endomicroscope. All measurements were expressed as the mean ± standard error. The mean diameter of tumor vessels was significantly larger than the normal vessels (9.46±0.4 µm vs. 7.60±0.3 µm, p = 0.0166). The vessel density was also significantly higher in the cancer vs. normal tissue samples (5541.05±262.81 vs. 3755.79±194.96 vessels/mm3, p = 0.0006). These results were confirmed by immunohistochemistry. In addition, the tortuosity index and vessel lengths were not significantly different (1.05±0.016 and 28.30±3.27 µm in normal tissue, vs. 1.07±0.008 and 26.49±3.18 µm in tumor tissue respectively, p = 0.5357 and p = 0.7033). The daughter/mother ratio (ratio of the sum of the squares of daughter vessel radii over the square of the mother vessel radius) was 1.15±0.09 in normal tissue, and 1.21±0.08 in tumor tissue (p = 0.6531). The confocal laser endomicroscopy is feasible for measuring more vascular parameters from fresh tumor biopsies than conventional immunohistochemistry alone. Provided new contrast agents will be clinically available, future in vivo use of CLE could lead to identification of novel biomarkers based on the morphometric characteristics of tumor vasculature.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0091084en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948726/pdf/en
dash.licenseLAAen_US
dc.subjectMedicineen
dc.subjectDiagnostic Medicineen
dc.subjectPathologyen
dc.subjectAnatomical Pathologyen
dc.subjectHistopathologyen
dc.subjectGastroenterology and Hepatologyen
dc.subjectColonen
dc.subjectColonoscopyen
dc.subjectOncologyen
dc.subjectBasic Cancer Researchen
dc.subjectTumor Physiologyen
dc.subjectCancer Detection and Diagnosisen
dc.subjectEarly Detectionen
dc.subjectCancer Treatmenten
dc.subjectAntiangiogenesis Therapyen
dc.subjectAntibody Therapyen
dc.subjectCancers and Neoplasmsen
dc.subjectGastrointestinal Tumorsen
dc.subjectColon Adenocarcinomaen
dc.subjectRectal Canceren
dc.titleEvaluation of New Morphometric Parameters of Neoangiogenesis in Human Colorectal Cancer Using Confocal Laser Endomicroscopy (CLE) and Targeted Panendothelial Markersen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorGruionu, Gabrielen_US
dc.date.available2014-04-11T14:12:11Z
dc.identifier.doi10.1371/journal.pone.0091084*
dash.authorsorderedfalse
dash.contributor.affiliatedGruionu, Gabriel


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