dc.contributor.author | Takeuchi, Kimio | en_US |
dc.contributor.author | Yanai, Ryoji | en_US |
dc.contributor.author | Kumase, Fumiaki | en_US |
dc.contributor.author | Morizane, Yuki | en_US |
dc.contributor.author | Suzuki, Jun | en_US |
dc.contributor.author | Kayama, Maki | en_US |
dc.contributor.author | Brodowska, Katarzyna | en_US |
dc.contributor.author | Nakazawa, Mitsuru | en_US |
dc.contributor.author | Miller, Joan W. | en_US |
dc.contributor.author | Connor, Kip M. | en_US |
dc.contributor.author | Vavvas, Demetrios G. | en_US |
dc.date.accessioned | 2014-04-11T14:12:38Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Takeuchi, K., R. Yanai, F. Kumase, Y. Morizane, J. Suzuki, M. Kayama, K. Brodowska, et al. 2014. “EGF-Like-Domain-7 Is Required for VEGF-Induced Akt/ERK Activation and Vascular Tube Formation in an Ex Vivo Angiogenesis Assay.” PLoS ONE 9 (3): e91849. doi:10.1371/journal.pone.0091849. http://dx.doi.org/10.1371/journal.pone.0091849. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:12064484 | |
dc.description.abstract | EGFL7 is a secreted angiogenic factor, which in contrast to the well-known secreted angiogenic molecules VEGF and FGF-2, is almost exclusively expressed by endothelial cells and may act in an autocrine fashion. Prior studies have shown EGFL7 to mediate its angiogenic effects by interfering with the Notch pathway and/or via the intronic miR126. Less is known about its effects on VEGF signaling. We wanted to investigate the role of epidermal growth factor-like domain 7 (EGFL7) in VEGF-driven angiogenesis using an ex vivo Matrigel-embedded mouse eye cup assay and siRNA mediated knockdown of EGFL7 by siRNA. Our results suggested that VEGF-induced vascular tube formation was significantly impaired after siRNA downregulation of EGFL7. In addition, knockdown of EGFL7 suppressed VEGF upregulation of phospho-Akt and phospho-Erk(1/2) in endothelial cells, but did not alter VEGFR phosphorylation and neuropilin-1 protein expression or miR126 expression. Thus, in conclusion, EGFL7 is required for VEGF upregulation of the Akt/Erk (1/2) pathway during angiogenesis, and may represent a new therapeutic target in diseases of pathological neovascularization. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0091849 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960138/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Biology and life sciences | en |
dc.subject | Anatomy | en |
dc.subject | Biological Tissue | en |
dc.subject | Epithelium | en |
dc.subject | Epithelial Cells | en |
dc.subject | Endothelial Cells | en |
dc.subject | Cell biology | en |
dc.subject | Signal transduction | en |
dc.subject | Cell signaling | en |
dc.subject | Signaling cascades | en |
dc.subject | AKT signaling cascade | en |
dc.subject | ERK signaling cascade | en |
dc.subject | Extracellular Matrix Signaling | en |
dc.subject | Membrane Receptor Signaling | en |
dc.subject | Cellular Types | en |
dc.subject | Molecular Cell Biology | en |
dc.subject | Medicine and Health Sciences | en |
dc.subject | Ophthalmology | en |
dc.subject | Pathology and Laboratory Medicine | en |
dc.subject | Molecular Pathology | en |
dc.subject | Vascular Medicine | en |
dc.subject | Vascular Diseases | en |
dc.subject | Peripheral Vascular Disease | en |
dc.subject | Model Organisms | en |
dc.subject | Animal Models | en |
dc.subject | Mouse Models | en |
dc.title | EGF-Like-Domain-7 Is Required for VEGF-Induced Akt/ERK Activation and Vascular Tube Formation in an Ex Vivo Angiogenesis Assay | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Kumase, Fumiaki | en_US |
dc.date.available | 2014-04-11T14:12:38Z | |
dc.identifier.doi | 10.1371/journal.pone.0091849 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Kumase, Fumiaki | |
dash.contributor.affiliated | Miller, Joan | |
dash.contributor.affiliated | Vavvas, Demetrios | |
dash.contributor.affiliated | Connor, Kip | |